MINT BIOTECHNOLOGIES CO., LTD. (20240240214). GENETICALLY ENGINEERED MICROORGANISM WITH HIGH YIELD OF L-ISOLEUCINE AND METHOD FOR PRODUCING L-ISOLEUCINE BY FERMENTATION simplified abstract

GENETICALLY ENGINEERED MICROORGANISM WITH HIGH YIELD OF L-ISOLEUCINE AND METHOD FOR PRODUCING L-ISOLEUCINE BY FERMENTATION

Organization Name

MINT BIOTECHNOLOGIES CO., LTD.

Inventor(s)

Kechun Zhang of Hangzhou, Zhejiang (CN)

Jingyu Wang of Hangzhou, Zhejiang (CN)

Jilong Wang of Hangzhou, Zhejiang (CN)

GENETICALLY ENGINEERED MICROORGANISM WITH HIGH YIELD OF L-ISOLEUCINE AND METHOD FOR PRODUCING L-ISOLEUCINE BY FERMENTATION - A simplified explanation of the abstract

This abstract first appeared for US patent application 20240240214 titled 'GENETICALLY ENGINEERED MICROORGANISM WITH HIGH YIELD OF L-ISOLEUCINE AND METHOD FOR PRODUCING L-ISOLEUCINE BY FERMENTATION

The method described in the patent application aims to increase the yield of l-isoleucine through fermentation using a genetically engineered strain.

• Genetic engineering is utilized to obtain a strain with specific genes that release the inhibition of l-isoleucine production.
• Fermentation culture is performed on the genetically engineered strain, with the addition of diketobutyric acid or a raw material that can be converted into it.
• The process includes separating l-isoleucine from the culture after fermentation.

Potential Applications: - Biotechnology industry for amino acid production - Pharmaceutical industry for drug synthesis - Food industry for enhancing nutritional content

Problems Solved: - Increasing the yield of l-isoleucine production - Overcoming inhibition factors in the fermentation process

Benefits: - Higher yield of l-isoleucine - Cost-effective production process - Enhanced efficiency in amino acid production

Commercial Applications: Title: "Enhanced L-Isoleucine Production Method for Biotechnology Industry" This technology can be utilized in the biotechnology industry for large-scale production of l-isoleucine, catering to pharmaceutical, food, and other related sectors.

Questions about the technology: 1. How does genetic engineering play a role in increasing l-isoleucine yield?

  Genetic engineering is used to obtain a strain with specific genes that release the inhibition of l-isoleucine production, leading to higher yields.

2. What are the potential applications of this technology in the pharmaceutical industry?

  This technology can be used in the pharmaceutical industry for synthesizing drugs that require l-isoleucine as a key component.

Original Abstract Submitted

a method for producing l-isoleucine at a higher yield by fermentation includes the step of using a genetic engineering method to obtain a genetically engineered strain. the genetically engineered strain has a threonine deaminase gene substantially releasing the inhibition of l-isoleucine and/or an acetylated hydroxy acid synthetase iii gene substantially releasing the inhibition of l-isoleucine; and performing fermentation culture on the genetically engineered strain, adding diketobutyric acid or a raw material capable of being converted into diketobutyric acid in a culture process, and separating l-isoleucine from a culture after the end of culturing. further provided is a genetically engineered strain for realizing high yield of l-isoleucine.