INBIOSE N.V. (20240417767). PRODUCTION OF ALPHA-1,3-FUCOSYLATED COMPOUNDS
Contents
PRODUCTION OF ALPHA-1,3-FUCOSYLATED COMPOUNDS
Organization Name
Inventor(s)
Joeri Beauprez of Zwijnaarde (BE)
Kristof Vandewalle of Zwijnaarde (BE)
Annelies Vercauteren of Zwijnaarde (BE)
PRODUCTION OF ALPHA-1,3-FUCOSYLATED COMPOUNDS
This abstract first appeared for US patent application 20240417767 titled 'PRODUCTION OF ALPHA-1,3-FUCOSYLATED COMPOUNDS
Original Abstract Submitted
this disclosure is in the technical field of synthetic biology, metabolic engineering and cell cultivation. the disclosure describes methods for the production of a fucosylated compound using a fucosyltransferase as well as the purification of the fucosylated compound, the fucosyltransferase having alpha-1,3-fucosyltransferase activity on the n-acetylglucosamine (glcnac) and/or the glucose (glc) residue of gal-�1,m-glcnac-�1,n-gal-�1,4-glc of a saccharide substrate comprising gal-�1,m-glcnac-�1,n-gal-�1,4-glc wherein the m is 3 or 4 and the n is 3 or 6. the disclosure also provides a cell for production of a fucosylated compound. next, the disclosure describes methods for the production of 3-fucosyllactose (3-fl) using a fucosyltransferase having alpha-1,3-fucosyltransferase activity on the glc residue of lactose, as well as the purification of the 3-fl. the disclosure also provides a cell for production of 3-fl.
