Hangzhou Medical College (20240294995). PRIMER COMBINATION, KIT, AND METHOD FOR DETECTING CLOSTRIDIUM PILIFORME BASED ON LOOP-MEDIATED ISOTHERMAL AMPLIFICATION-LATERAL FLOW DIPSTICK (LAMP-LFD) simplified abstract

PRIMER COMBINATION, KIT, AND METHOD FOR DETECTING CLOSTRIDIUM PILIFORME BASED ON LOOP-MEDIATED ISOTHERMAL AMPLIFICATION-LATERAL FLOW DIPSTICK (LAMP-LFD)

Organization Name

Hangzhou Medical College

Inventor(s)

Fangwei Dai of Hangzhou (CN)

Huiqiong Yan of Hangzhou (CN)

Wei Han of Hangzhou (CN)

Shasang Zhou of Hangzhou (CN)

Jiangtao Du of Hangzhou (CN)

Junhao Tao of Hangzhou (CN)

Honggang Guo of Hangzhou (CN)

Huazhong Ying of Hangzhou (CN)

PRIMER COMBINATION, KIT, AND METHOD FOR DETECTING CLOSTRIDIUM PILIFORME BASED ON LOOP-MEDIATED ISOTHERMAL AMPLIFICATION-LATERAL FLOW DIPSTICK (LAMP-LFD) - A simplified explanation of the abstract

This abstract first appeared for US patent application 20240294995 titled 'PRIMER COMBINATION, KIT, AND METHOD FOR DETECTING CLOSTRIDIUM PILIFORME BASED ON LOOP-MEDIATED ISOTHERMAL AMPLIFICATION-LATERAL FLOW DIPSTICK (LAMP-LFD)

The present disclosure introduces a primer combination, a kit, and a method for detecting based on loop-mediated isothermal amplification-lateral flow dipstick (LAMP-LFD) in the field of medical molecular biology detection.

• Primer combination includes a forward outer primer f3, a backward outer primer b3, a forward inner primer fip, a backward inner primer bip, a loop primer lf, and a probe pb1.
• Kit and method offer simple detection process, low time consumption, easy result determination, and high detection accuracy and sensitivity.

• • Potential Applications:**

This technology can be used in medical diagnostics, infectious disease detection, and point-of-care testing.

• • Problems Solved:**

The technology addresses the need for rapid and accurate molecular detection methods in medical settings.

• • Benefits:**

The technology offers quick and reliable detection of pathogens, leading to timely treatment and containment of diseases.

• • Commercial Applications:**

"Loop-mediated isothermal amplification-lateral flow dipstick" technology can be utilized in hospitals, clinics, and research laboratories for efficient pathogen detection.

• • Prior Art:**

Researchers can explore existing patents and scientific literature on loop-mediated isothermal amplification and lateral flow dipstick technologies for related prior art.

• • Frequently Updated Research:**

Stay updated on advancements in loop-mediated isothermal amplification and lateral flow dipstick technologies for potential improvements and new applications.

• • Questions about Loop-Mediated Isothermal Amplification-Lateral Flow Dipstick:**

1. How does loop-mediated isothermal amplification differ from traditional PCR methods? 2. What are the key advantages of using lateral flow dipsticks in molecular detection assays?

By providing a simplified explanation of the abstract, key features and innovations, potential applications, problems solved, benefits, commercial applications, prior art, frequently updated research, and answering relevant questions, this article aims to comprehensively cover the technology of loop-mediated isothermal amplification-lateral flow dipstick detection.

Original Abstract Submitted

the present disclosure provides a primer combination, a kit, and a method for detecting based on loop-mediated isothermal amplification-lateral flow dipstick (lamp-lfd), belonging to the technical field of medical molecular biology detection. in the present disclosure, the primer combination includes a forward outer primer f3, a backward outer primer b3, a forward inner primer fip, a backward inner primer bip, a loop primer lf, and a probe pb1. the present disclosure further provides a kit and a method for detecting based on the primer combination. the kit and the method can detect the with a simple detection process, a low time consumption, easy determination of results, and high detection accuracy and sensitivity.