Detect, Inc. (20240336954). AUTOMATED DESIGN OF PRIMER SETS FOR NUCLEIC ACID AMPLIFICATION simplified abstract

AUTOMATED DESIGN OF PRIMER SETS FOR NUCLEIC ACID AMPLIFICATION

Organization Name

Detect, Inc.

Inventor(s)

Jakob T. Nielsen of Tilst (DE)

Mary O'neill of Seattle WA (US)

Simon Chardin of Paris (FR)

Henry Kemble of Paris (FR)

Spencer Glantz of West Hartford CT (US)

Ben Goldstein of Westport CT (US)

Matt Higgins of London (GB)

Morten Schaumburg of Aarhus V. (DE)

Eric Kauderer-abrams of Redwood City CA (US)

AUTOMATED DESIGN OF PRIMER SETS FOR NUCLEIC ACID AMPLIFICATION - A simplified explanation of the abstract

This abstract first appeared for US patent application 20240336954 titled 'AUTOMATED DESIGN OF PRIMER SETS FOR NUCLEIC ACID AMPLIFICATION

The patent application describes methods for determining a primer set for amplifying a target nucleic acid, along with apparatuses and computer-readable storage media configured to perform these methods.

• Obtaining a first primer set
• Generating multiple child primer sets by modifying the first primer set
• Determining a fitness score for each child primer set
• Adding acceptable child primer sets to a collection stored in memory
• Generating child primer sets in parallel
• Using a genetic algorithm to control various aspects of the methods

Potential Applications: - Genetic testing - Disease diagnosis - Forensic analysis

Problems Solved: - Streamlining primer set selection process - Improving accuracy of nucleic acid amplification

Benefits: - Increased efficiency in nucleic acid amplification - Enhanced accuracy in genetic testing

Commercial Applications: Title: "Next-Generation Nucleic Acid Amplification Technology" This technology could be used in medical laboratories, research institutions, and biotechnology companies for various applications such as genetic testing, disease diagnosis, and forensic analysis.

Questions about Nucleic Acid Amplification Technology: 1. How does this technology improve the accuracy of genetic testing? 2. What are the potential drawbacks of using a genetic algorithm to control primer set selection?

Original Abstract Submitted

methods are provided for determining a primer set for amplifying a target nucleic acid, as well as apparatuses and computer-readable storage media configured to perform aspects of the methods. in some cases, the methods include obtaining a first primer set; generating multiple child primer sets by performing modifications to the first primer set; and, for each of the child primer sets, determining a fitness score of the child primer set and, if the fitness score is at or above a predetermined threshold, determining the child primer set to be an acceptable primer set and adding the child primer set to a collection of acceptable primer sets stored in a memory device. the generating may generate at least some of the child primer sets in parallel. multiple collections of acceptable primer sets may be generated in parallel. various aspects of the methods may be controlled by a genetic algorithm.