20240041922. METHODS AND COMPOSITIONS simplified abstract (Monash University)
Contents
METHODS AND COMPOSITIONS
Organization Name
Inventor(s)
Dhanushika Ratnayake of Clayton, Victoria (AU)
Peter Currie of Clayton, Victoria (AU)
Mikael Martino of Clayton, Victoria (AU)
METHODS AND COMPOSITIONS - A simplified explanation of the abstract
This abstract first appeared for US patent application 20240041922 titled 'METHODS AND COMPOSITIONS
Simplified Explanation
The patent application describes methods of stimulating muscle regeneration by delivering a CCR5 interacting agent or encoding molecule to a muscle. The CCR5 interacting agent binds to muscle stem cells and stimulates myoblast proliferation and muscle regeneration. One example of the CCR5 interacting agent is nampt, which includes a cytokine finger motif or a derivative thereof. The application also includes cellular compositions that express the CCR5 interacting agent, such as a population of satellite or macrophage cells or their precursors/progeny. These compositions can be used in stem cell therapy or for treating muscular deficiencies, disorders, or injuries. The examples provided in the application demonstrate muscle tissue regeneration with minimal fibrosis. Additionally, a nampt polypeptide fragment comprising a C-terminal portion of nampt, including a cytokine finger, is enabled. Compositions further comprise the nampt polypeptide fragment and one or more of tissue stem cells or macrophages or their precursor/progeny, scaffold or a retentive material, and tissue delivery enhancing or cell retention moiety.
- Methods of stimulating muscle regeneration by delivering a CCR5 interacting agent or encoding molecule to a muscle
- The CCR5 interacting agent binds to muscle stem cells and stimulates myoblast proliferation and muscle regeneration
- Nampt is an example of a CCR5 interacting agent, which includes a cytokine finger motif or a derivative thereof
- Cellular compositions expressing the CCR5 interacting agent, such as satellite or macrophage cells or their precursors/progeny, are included
- These compositions can be used in stem cell therapy or for treating muscular deficiencies, disorders, or injuries
- Examples demonstrate muscle tissue regeneration with minimal fibrosis
- A nampt polypeptide fragment comprising a C-terminal portion of nampt, including a cytokine finger, is enabled
- Compositions further comprise the nampt polypeptide fragment and one or more of tissue stem cells or macrophages or their precursor/progeny, scaffold or a retentive material, and tissue delivery enhancing or cell retention moiety
Potential Applications
- Stem cell therapy
- Treatment of muscular deficiencies, disorders, or injuries
Problems Solved
- Stimulating muscle regeneration
- Promoting myoblast proliferation
- Minimizing fibrosis in muscle tissue regeneration
Benefits
- Enhanced muscle regeneration
- Improved treatment of muscular deficiencies, disorders, or injuries
- Reduced fibrosis in muscle tissue regeneration
Original Abstract Submitted
methods of stimulating muscle regeneration, comprising delivering to a muscle a ccr5 interacting agent or encoding molecule. the ccr5 interacting agent binds to muscle stem cells and stimulates myoblast proliferation and muscle regeneration. one example of the ccr5 interacting agent is nampt comprising a cytokine finger motif or a derivative thereof. methods and compositions include cellular compositions, which expresses the ccr5 interacting agent; including a population of satellite or macrophage cells or their precursors/progeny and their applications in stem cell therapy or for use in treating a muscular deficiency, disorder or injury. the examples show muscle tissue regeneration with minimal fibrosis. also enabled is a nampt polypeptide fragment comprising a c-terminal portion of nampt comprising a cytokine finger. compositions further comprise the nampt polypeptide fragment and one or more of; tissue stem cell or macrophage or precursor/progeny, scaffold or a retentive material, tissue delivery enhancing or cell retention moiety.
