18972398. DEAMINASE-BASED RNA SENSORS (Massachusetts Institute of Technology)
DEAMINASE-BASED RNA SENSORS
Organization Name
Massachusetts Institute of Technology
Inventor(s)
KAIYI Jiang of CAMBRIDGE MA US
ROHAN NEIL Krajeski of BOSTON MA US
OMAR OSAMA Abudayyeh of CAMBRIDGE MA US
JONATHAN S. Gootenberg of CAMBRIDGE MA US
YIFAN Zhang of CAMBRIDGE MA US
XI Chen of ROXBURY CROSSING MA US
JEREMY G. Koob of SOMERVILLE MA US
DEAMINASE-BASED RNA SENSORS
This abstract first appeared for US patent application 18972398 titled 'DEAMINASE-BASED RNA SENSORS
Original Abstract Submitted
RNA editing tools for use in systems designed to measure RNA in vivo and manipulate specific cell types are disclosed herein. An RNA sensor system comprising a) a single-stranded RNA (ssRNA) sensor comprising a stop codon and a payload; optionally wherein the ssRNA sensor further comprises a normalizing gene; and b) an adenosine deaminase acting on RNA (ADAR) deaminase; wherein the sensor is capable of binding to a ssRNA target to form a double-stranded RNA (dsRNA) duplex that becomes a substrate for the ADAR deaminase; wherein the substrate comprises a mispairing within the stop codon; and wherein the mispairing is editable by the ADAR deaminase, which editing can effectively remove the stop codon so as to enable translation and expression of the payload. A method of quantifying ribonucleic acid (RNA) levels using the RNA sensor system is also disclosed.
- Massachusetts Institute of Technology
- KAIYI Jiang of CAMBRIDGE MA US
- ROHAN NEIL Krajeski of BOSTON MA US
- OMAR OSAMA Abudayyeh of CAMBRIDGE MA US
- JONATHAN S. Gootenberg of CAMBRIDGE MA US
- YIFAN Zhang of CAMBRIDGE MA US
- FEI Chen of CAMBRIDGE MA US
- XI Chen of ROXBURY CROSSING MA US
- JEREMY G. Koob of SOMERVILLE MA US
- C12N15/113
- C12N9/50
- C12N9/78
- C12N15/10
- C12Q1/6897
- CPC C12N15/113