18820404. OPTICAL DISTORTION CORRECTION FOR IMAGED SAMPLES (ILLUMINA, INC.)
Contents
OPTICAL DISTORTION CORRECTION FOR IMAGED SAMPLES
Organization Name
Inventor(s)
Robert Langlois of San Diego CA (US)
Paul Belitz of San Diego CA (US)
OPTICAL DISTORTION CORRECTION FOR IMAGED SAMPLES
This abstract first appeared for US patent application 18820404 titled 'OPTICAL DISTORTION CORRECTION FOR IMAGED SAMPLES
Original Abstract Submitted
Techniques are described for dynamically correcting image distortion during imaging of a patterned sample having repeating spots. Different sets of image distortion correction coefficients may be calculated for different regions of a sample during a first imaging cycle of a multicycle imaging run and subsequently applied in real time to image data generated during subsequent cycles. In one implementation, image distortion correction coefficients may be calculated for an image of a patterned sample having repeated spots by: estimating an affine transform of the image; sharpening the image; and iteratively searching for an optimal set of distortion correction coefficients for the sharpened image, where iteratively searching for the optimal set of distortion correction coefficients for the sharpened image includes calculating a mean chastity for spot locations in the image, and where the estimated affine transform is applied during each iteration of the search.
