18677250. ANALYSIS METHOD simplified abstract (DENSO CORPORATION)

ANALYSIS METHOD

Organization Name

DENSO CORPORATION

Inventor(s)

Akira Nukazuka of Kariya-City (JP)

Teppei Sakai of Kariya-City (JP)

Mana Asano of Kariya-City (JP)

Kei Hayakawa of Kariya-City (JP)

Kazuhisa Nakagawa of Kariya-City (JP)

Mai Niimoto of Kariya-City (JP)

ANALYSIS METHOD - A simplified explanation of the abstract

This abstract first appeared for US patent application 18677250 titled 'ANALYSIS METHOD

Simplified Explanation

The analysis method described in the patent application involves mixing a binding substance with a sample containing a target substance, removing unbound binding substance, amplifying a nucleic acid region, and detecting the amplified nucleic acid region.

• The method includes forming a complex with a template nucleic acid and amplifying the nucleic acid region using a nucleic acid amplifying enzyme.
• The binding substance contains a nucleic acid region that binds to the target substance.
• The phenomenon caused by the amplification of the nucleic acid region is detected.

Key Features and Innovation

• Mixing a binding substance with a sample to detect a target substance.
• Amplifying a nucleic acid region to enhance detection sensitivity.
• Using a nucleic acid amplifying enzyme to amplify the nucleic acid region.
• Forming a complex with a template nucleic acid to facilitate amplification.
• Detecting a phenomenon caused by the amplification of the nucleic acid region.

Potential Applications

The technology can be used in various fields such as medical diagnostics, environmental testing, and food safety to detect specific target substances with high sensitivity and accuracy.

Problems Solved

The technology addresses the need for sensitive and specific detection methods for target substances in various samples.

Benefits

• High sensitivity and accuracy in detecting target substances.
• Efficient and reliable analysis method.
• Versatile applications in different industries.

Commercial Applications

The technology can be applied in medical laboratories, environmental monitoring agencies, and food safety organizations for rapid and accurate detection of target substances.

Prior Art

Readers can explore prior research on nucleic acid amplification techniques, binding substances for target detection, and complex formation in nucleic acid analysis.

Frequently Updated Research

Researchers are continually improving nucleic acid amplification methods, enhancing binding substances' specificity, and developing novel detection techniques for target substances.

Questions about Nucleic Acid Analysis

What are the potential limitations of using nucleic acid amplification in target detection?

Nucleic acid amplification techniques can be sensitive to contamination and require careful handling to prevent false positives.

How can the specificity of binding substances be improved for accurate target detection?

Researchers are exploring modifications to binding substances to enhance their specificity and reduce non-specific binding.

Original Abstract Submitted

An analysis method according to the present disclosure includes: mixing a binding substance and a sample including a target substance. The binding substance includes a nucleic acid region composed of a nucleic acid and having activity to bind to the target substance; removing the binding substance not bound to the target substance; amplifying the nucleic acid region; and detecting a phenomenon caused by the amplification of the nucleic acid region. For example, the analysis method includes: forming a complex of a template nucleic acid including a sequence complementary to a 3′ side sequence of the nucleic acid region; and amplifying the nucleic acid region by an action of a nucleic acid amplifying enzyme using the complex as a starting point.