18652258. METHOD OF DETECTING TARGET SUBSTANCE simplified abstract (TOPPAN Holdings Inc.)
Contents
- 1 METHOD OF DETECTING TARGET SUBSTANCE
- 1.1 Organization Name
- 1.2 Inventor(s)
- 1.3 METHOD OF DETECTING TARGET SUBSTANCE - A simplified explanation of the abstract
- 1.4 Simplified Explanation
- 1.5 Key Features and Innovation
- 1.6 Potential Applications
- 1.7 Problems Solved
- 1.8 Benefits
- 1.9 Commercial Applications
- 1.10 Questions about Target Substance Detection
- 1.11 Original Abstract Submitted
METHOD OF DETECTING TARGET SUBSTANCE
Organization Name
Inventor(s)
Yoichi Makino of Taito-ku (JP)
METHOD OF DETECTING TARGET SUBSTANCE - A simplified explanation of the abstract
This abstract first appeared for US patent application 18652258 titled 'METHOD OF DETECTING TARGET SUBSTANCE
Simplified Explanation
The patent application describes a method for detecting a target substance in a sample by using specific binding substances labeled with nucleic acid fragments and detecting the formation of double-stranded nucleic acid.
- Introducing sample, specific binding substances, and labeled nucleic acid fragments into wells
- Forming a complex if the target substance is present in the sample
- Hybridizing the labeled nucleic acid fragments to form double-stranded nucleic acid
- Detecting the presence of the target substance based on the formation of double-stranded nucleic acid
Key Features and Innovation
- Utilizes specific binding substances labeled with nucleic acid fragments for target substance detection
- Formation of double-stranded nucleic acid as an indicator of target substance presence
Potential Applications
- Medical diagnostics
- Environmental testing
- Food safety analysis
Problems Solved
- Rapid and accurate detection of target substances in samples
- Enhanced sensitivity and specificity in detection methods
Benefits
- Improved efficiency in target substance detection
- Early identification of harmful substances in various samples
Commercial Applications
- Diagnostic testing kits for healthcare facilities
- Quality control tools for food and beverage industries
Questions about Target Substance Detection
How does the method improve upon existing detection techniques?
The method enhances detection sensitivity and specificity by utilizing specific binding substances labeled with nucleic acid fragments.
What are the potential limitations of this detection method?
Potential limitations may include the need for specialized equipment and expertise for implementation.
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Original Abstract Submitted
A method of detecting a target substance in a sample includes introducing into wells the sample, a first specific binding substance which is for the target substance and labeled with a first single-stranded nucleic acid fragment, and a second specific binding substance which is for the target substance and labeled with a second single-stranded nucleic acid fragment, forming as a result a complex containing the target substance, the first specific binding substance, and the second specific binding substance if the target substance is present in the sample, and forming a double-stranded nucleic acid by hybridization of at least part of the first single-stranded nucleic acid fragment with at least part of the second single-stranded nucleic acid fragment; and detecting formation of the double-stranded nucleic acid, wherein detection of formation of the double-stranded nucleic acid indicates presence of the target substance.
