18406279. SYSTEMS AND METHODS OF DELIVERING TARGET MOLECULES TO A NANOPORE simplified abstract (The Regents of the University of California)
Contents
SYSTEMS AND METHODS OF DELIVERING TARGET MOLECULES TO A NANOPORE
Organization Name
The Regents of the University of California
Inventor(s)
Holger Schmidt of Capitola CA (US)
Aaron Roe Hawkins of Provo UT (US)
David W. Deamer of Santa Cruz CA (US)
SYSTEMS AND METHODS OF DELIVERING TARGET MOLECULES TO A NANOPORE - A simplified explanation of the abstract
This abstract first appeared for US patent application 18406279 titled 'SYSTEMS AND METHODS OF DELIVERING TARGET MOLECULES TO A NANOPORE
The disclosed system utilizes modulations of ionic current across a nanopore in a membrane to detect target molecules passing through the nanopore, with applications in nucleic acid sequencing and detection of proteins and small molecules.
- The system delivers target molecules to a nanopore for label-free single molecule analysis using a chip-based system.
- Target molecules are concentrated on microscale carrier beads, delivered to the nanopore, and released for detection using nanopore current modulation.
- Sample preparation processes such as purification, extraction, and pre-concentration are combined with nanopore-based readout on a microfluidic chip.
- Target molecules can be specifically bound to carrier beads and positioned within the capture volume of a nanopore using a chip-based microfluidic platform.
Potential Applications: - Nucleic acid sequencing - Protein and small molecule detection - Single molecule analysis
Problems Solved: - Enables label-free single molecule analysis - Provides a chip-based system for nanopore detection - Integrates sample preparation with nanopore-based readout
Benefits: - Enhanced sensitivity and specificity in molecular detection - Streamlined sample preparation processes - Potential for high-throughput analysis
Commercial Applications: Title: Nanopore-Based Molecular Detection System for Biomedical Research and Diagnostics This technology has potential commercial applications in biomedical research, pharmaceutical development, and clinical diagnostics. It can be used for rapid and accurate analysis of nucleic acids, proteins, and small molecules, making it valuable in various industries.
Prior Art: Prior art related to this technology may include existing nanopore sequencing systems, microfluidic devices for molecular analysis, and methods for single molecule detection.
Frequently Updated Research: Ongoing research in this field may focus on improving the sensitivity and throughput of the system, optimizing sample preparation processes, and expanding the range of molecular targets that can be detected using nanopore-based technology.
Questions about Nanopore-Based Molecular Detection System: 1. How does the system achieve label-free single molecule analysis? 2. What are the key advantages of using a chip-based microfluidic platform for nanopore detection?
Original Abstract Submitted
A disclosed system uses modulations of ionic current across a nanopore in a membrane to detect target molecules passing through the nanopore. This principle has been applied mainly to nucleic acid sequencing, but can also be used to detect other molecular targets such as proteins and small molecules. In addition, the system delivers target molecules to a nanopore to provide label-free single molecule analysis using a chip-based system. Target molecules are concentrated on microscale carrier beads, and the beads are delivered and optically trapped in an area within the capture radius of the nanopore. The target molecules are released from the beads and detected using nanopore current modulation. In addition, the disclosed system combines sample preparation (e.g. purification, extraction, and pre-concentration) with nanopore-based readout on a microfluidic chip. Finally, target molecules can be specifically bound to carrier beads and particles are positioned within the capture volume of a nanopore using a chip-based microfluidic platform proven to handle specific detection of molecular targets from milliliters of raw sample.
