18281188. PH-ACTIVABLE FLUORESCENT PROBES FOR TARGETING CELL ORGANELLES simplified abstract (PURDUE RESEARCH FOUNDATION)
Contents
- 1 PH-ACTIVABLE FLUORESCENT PROBES FOR TARGETING CELL ORGANELLES
- 1.1 Organization Name
- 1.2 Inventor(s)
- 1.3 PH-ACTIVABLE FLUORESCENT PROBES FOR TARGETING CELL ORGANELLES - A simplified explanation of the abstract
- 1.4 Simplified Explanation
- 1.5 Potential Applications
- 1.6 Problems Solved
- 1.7 Benefits
- 1.8 Potential Commercial Applications
- 1.9 Possible Prior Art
- 1.10 Original Abstract Submitted
PH-ACTIVABLE FLUORESCENT PROBES FOR TARGETING CELL ORGANELLES
Organization Name
Inventor(s)
Gaurav Chopra of West Lafayette IN (US)
Krupal Jethava of West Lafayette IN (US)
Priya Prakash of West Lafayette IN (US)
PH-ACTIVABLE FLUORESCENT PROBES FOR TARGETING CELL ORGANELLES - A simplified explanation of the abstract
This abstract first appeared for US patent application 18281188 titled 'PH-ACTIVABLE FLUORESCENT PROBES FOR TARGETING CELL ORGANELLES
Simplified Explanation
The present disclosure describes series pH-activable fluorescent probes based on a single BODIPY scaffold selectively targeting lysosomal, mitochondrial, and nucleus. The divergent cell organelle targeting was achieved by synthesizing pH-activable fluorescent probes with differential fluorescence profiles arising due to the presence of a unique functional group in the scaffold. We discovered that the functional group transformation in the same scaffold influences the localization ability of pH-activable fluorescent probes in cell organelles. The development of pH-activable fluorescent probes that target lysosomes and mitochondria organelles in live and fixed primary mouse microglial cells warrants future use in disease-specific biological models.
- pH-activable fluorescent probes based on a single BODIPY scaffold
- Selective targeting of lysosomal, mitochondrial, and nucleus organelles
- Differential fluorescence profiles due to unique functional group in the scaffold
- Functional group transformation influences localization ability in cell organelles
- Potential use in disease-specific biological models
Potential Applications
The technology can be applied in:
- Cell biology research
- Drug development for diseases affecting lysosomes and mitochondria
- Live cell imaging studies
Problems Solved
The technology addresses the following issues:
- Targeted delivery of fluorescent probes to specific cell organelles
- Monitoring cellular processes in real-time
- Studying disease mechanisms at the organelle level
Benefits
The benefits of this technology include:
- Enhanced understanding of cellular processes
- Improved visualization of organelle dynamics
- Potential for developing targeted therapies for organelle-related diseases
Potential Commercial Applications
The technology has potential commercial applications in:
- Pharmaceutical companies for drug development
- Research institutions for cell biology studies
- Biotechnology companies for imaging technologies
Possible Prior Art
One possible prior art could be the development of pH-activable fluorescent probes targeting specific organelles, but the use of a single BODIPY scaffold with differential fluorescence profiles based on a unique functional group may be a novel approach in this field.
Unanswered Questions
How does the presence of a unique functional group in the scaffold affect the fluorescence profiles of the probes?
The abstract mentions differential fluorescence profiles due to the unique functional group, but the specific mechanism behind this phenomenon is not explained.
What are the potential limitations or challenges in using these pH-activable fluorescent probes in disease-specific biological models?
While the abstract highlights the future use of the probes in disease-specific biological models, it does not mention any potential limitations or challenges that may arise in practical applications.
Original Abstract Submitted
The present disclosure describes series pH-activable fluorescent probes based on a single BODIPY scaffold selectively targeting lysosomal, mitochondrial, and nucleus. The divergent cell organelle targeting was achieved by synthesizing pH-activable fluorescent probes with differential fluorescence profiles arising due to the presence of a unique functional group in the scaffold. We discovered that the functional group transformation in the same scaffold influences the localization ability of pH-activable fluorescent probes in cell organelle. The development of pH-activable fluorescent probes that target lysosomes and mitochondria organelles in live and fixed primary mouse microglial cells warrants future use in disease-specific biological models.
