Zhejiang University (20240262891). HYBRIDOMA CELL LINE FOR SECRETING ANTI-RABIES VIRUS M PROTEIN MONOCLONAL ANTIBODY AND APPLICATION THEREOF simplified abstract

HYBRIDOMA CELL LINE FOR SECRETING ANTI-RABIES VIRUS M PROTEIN MONOCLONAL ANTIBODY AND APPLICATION THEREOF

Organization Name

Zhejiang University

Inventor(s)

Jiyoung Zhou of Hangzhou, Zhejiang (CN)

Min Liao of Hangzhou, Zhejiang (CN)

Yan Yan of Hangzhou, Zhejiang (CN)

Boli Hu of Hangzhou, Zhejiang (CN)

Jinyan Gu of Hangzhou, Zhejiang (CN)

Xiaojuan Zheng of Hangzhou, Zhejiang (CN)

Weiren Dong of Hangzhou, Zhejiang (CN)

Yulan Jin of Hangzhou, Zhejiang (CN)

HYBRIDOMA CELL LINE FOR SECRETING ANTI-RABIES VIRUS M PROTEIN MONOCLONAL ANTIBODY AND APPLICATION THEREOF - A simplified explanation of the abstract

This abstract first appeared for US patent application 20240262891 titled 'HYBRIDOMA CELL LINE FOR SECRETING ANTI-RABIES VIRUS M PROTEIN MONOCLONAL ANTIBODY AND APPLICATION THEREOF

The present invention discloses a hybridoma cell line for secreting monoclonal antibody against rabies virus M protein and its application in the field of biotechnology. The hybridoma cell line, named as hybridoma cell line 4a1, was deposited on Apr. 1, 2019, in the China Center for Type Culture Collection, Wuhan University, Wuhan, China, with a deposit number CCTCC No: C201947. The monoclonal antibody produced by this cell line exhibits high titer, good specificity, and excellent biological characteristics. This invention identifies the variant antigen epitope recognized by the rabies virus M protein, enabling the hybridoma cell line to distinguish the Flury strain and other rabies virus strains, prepare kits for detecting rabies virus, detect rabies virus infection, and differentially diagnose vaccines for the Flury strain and other rabies virus strains.

• High titer, good specificity, and excellent biological characteristics of the monoclonal antibody
• Ability to distinguish Flury strain and other rabies virus strains
• Preparation of kits for detecting rabies virus and differential diagnosis of vaccines
• Identification of variant antigen epitope recognized by rabies virus M protein
• Application in detecting rabies virus infection

• • Potential Applications:**

The technology can be applied in the development of diagnostic kits for detecting rabies virus, differential diagnosis of rabies virus strains, and vaccine development.

• • Problems Solved:**

The technology addresses the need for accurate detection and differentiation of rabies virus strains, as well as the development of effective vaccines.

• • Benefits:**

The technology offers high specificity, sensitivity, and accuracy in detecting rabies virus infections, aiding in timely diagnosis and treatment.

• • Commercial Applications:**

The technology can be commercialized in the biotechnology and pharmaceutical industries for the development of diagnostic kits, vaccines, and research tools for rabies virus detection and differentiation.

• • Questions about the Technology:**

1. How does the hybridoma cell line 4a1 contribute to the accurate detection of rabies virus strains? 2. What are the potential commercial applications of the monoclonal antibody produced by the hybridoma cell line?

Original Abstract Submitted

the present invention discloses a hybridoma cell line for secreting monoclonal antibody against rabies virus m protein and application thereof, relates to the field of biotechnology. a classification of the hybridoma cell line is named as hybridoma cell line 4a1, and the hybridoma cell line was deposited on apr. 1, 2019 in the china center for type culture collection, wuhan university, wuhan, china, with a deposit number cctcc no: c201947. the monoclonal antibody prepared by the hybridoma cell line has high titer, good specificity and excellent biological characteristics. the present invention identifies the variant antigen epitope recognized by the rabv m protein, the hybridoma cell line can be used to distinguish flury strain and other rabv strains, prepare kit for detecting rabies virus rabv, detect rabv infection and differential diagnosis vaccine flury strain and other rabv strains.