Life Technologies Corporation (20240247286). METHODS FOR IMPROVED HOMOLOGOUS RECOMBINATION AND COMPOSITIONS THEREOF simplified abstract
Contents
METHODS FOR IMPROVED HOMOLOGOUS RECOMBINATION AND COMPOSITIONS THEREOF
Organization Name
Inventor(s)
Xiquan Liang of San Diego CA (US)
Robert Jason Potter of San Marcos CA (US)
METHODS FOR IMPROVED HOMOLOGOUS RECOMBINATION AND COMPOSITIONS THEREOF - A simplified explanation of the abstract
This abstract first appeared for US patent application 20240247286 titled 'METHODS FOR IMPROVED HOMOLOGOUS RECOMBINATION AND COMPOSITIONS THEREOF
The present disclosure pertains to methods, kits, and compositions for enhancing the efficiency of homologous recombination, particularly focusing on cloning DNA molecules directly into a genome using promoter trapping, short homology arms, nuclear localization signal, and DNA binding agents like TAL effector domain or truncated guide RNA bound by Cas9 to specific sites to modify chromatin structure and increase accessibility for enzymatic modifications.
- Methods for cloning DNA directly into a genome
- Use of promoter trapping and short homology arms
- Utilization of nuclear localization signal and DNA binding agents
- Displacement or restructuring of chromatin at target locus
- Increased accessibility of target locus for enzymatic modifications
Potential Applications: - Genome editing - Enhancing enzymatic processes
Problems Solved: - Improving efficiency of homologous recombination - Facilitating direct cloning of DNA into genomes
Benefits: - Precision in genome editing - Enhanced efficiency of enzymatic processes
Commercial Applications: Title: Enhanced Genome Editing Technology for Biotechnology Industry Description: This technology can be utilized in biotechnology companies for precise genome editing and improving enzymatic processes, leading to the development of novel products and therapies.
Questions about Enhanced Genome Editing Technology: 1. How does the combined use of promoter trapping and short homology arms improve the efficiency of homologous recombination? 2. What are the potential implications of utilizing DNA binding agents like TAL effector domain or Cas9 in genome editing processes?
Original Abstract Submitted
the present disclosure relates to methods, kits, and compositions for improving the efficiency of homologous recombination. in particular, the disclosure relates to methods for cloning dna molecules directly into a genome with the combined use of promoter trapping and short homology arms, nuclear localization signal, and/or binding one or more dna binding agents (tal effector domain or truncated guide rna bound by cas9) to specific sites thereby displacing or restructuring chromatin at the target locus, and/or it increasing the accessibility of the target locus to further enzymatic modifications. the methods and compositions provided herein are, inter alia, useful for genome editing and enhancing enzymatic processes involved therein.
