Guangzhou SequMed Biology Technology Co., Ltd. (20240229125). METHOD FOR MEDIUM-THROUGHPUT MULTI-SINGLE-CELL REPRESENTATIVE DNA METHYLATION LIBRARY CONSTRUCTION AND SEQUENCING simplified abstract
METHOD FOR MEDIUM-THROUGHPUT MULTI-SINGLE-CELL REPRESENTATIVE DNA METHYLATION LIBRARY CONSTRUCTION AND SEQUENCING
Organization Name
Guangzhou SequMed Biology Technology Co., Ltd.
Inventor(s)
METHOD FOR MEDIUM-THROUGHPUT MULTI-SINGLE-CELL REPRESENTATIVE DNA METHYLATION LIBRARY CONSTRUCTION AND SEQUENCING - A simplified explanation of the abstract
This abstract first appeared for US patent application 20240229125 titled 'METHOD FOR MEDIUM-THROUGHPUT MULTI-SINGLE-CELL REPRESENTATIVE DNA METHYLATION LIBRARY CONSTRUCTION AND SEQUENCING
The patent application describes a set of adhesive adapters with sample barcodes for tagging different samples and a method for detecting CPG methylation in multiple samples simultaneously through multi-sample reduced-representation bisulfite sequencing (MSRRBS) or multi-sample reduced-representation APOBEC sequencing (MSRRAS).
- Adhesive adapters with sample barcodes for specific sample tagging
- Simultaneous detection of CPG methylation in multiple samples using MSRRBS or MSRRAS
- Pooling of samples for a single-tube reaction after tagging
- Subsequent conversion, library construction, sequencing, distribution, decoding, and downstream analysis of samples
- High efficiency, low cost, stable, and convenient library construction technology
- Potential Applications:**
- Research in epigenetics and DNA methylation studies - Clinical diagnostics for detecting methylation patterns in diseases - Forensic analysis for sample identification and profiling
- Problems Solved:**
- Efficient and cost-effective method for detecting methylation in multiple samples - Simplified workflow for processing a large number of samples simultaneously - Enhanced sample tracking and data analysis through sample barcoding
- Benefits:**
- Increased throughput and efficiency in methylation analysis - Cost savings due to pooled sample processing - Improved accuracy and reliability in sample identification and analysis
- Commercial Applications:**
- Title:** High-Throughput Methylation Analysis Technology for Research and Diagnostics
- Description:** This technology can be utilized in research laboratories, diagnostic centers, and forensic facilities for high-throughput methylation analysis, offering a competitive advantage in terms of speed, cost, and accuracy.
- Questions about the Technology:**
1. How does the use of adhesive adapters with sample barcodes improve the efficiency of sample tagging? 2. What are the advantages of using multi-sample reduced-representation sequencing methods like MSRRBS and MSRRAS for methylation analysis?
Original Abstract Submitted
disclosed is a set of adhesive adapters containing sample barcodes for specifically tagging different samples. further disclosed is a method for simultaneously detecting cpg methylation in a high number of samples, which is multi-sample reduced-representation bisulfite sequencing (msrrbs); and an alternative method thereof, which is multi-sample reduced-representation apobec sequencing (msrras). the adapters are used to specifically tag the plurality of samples, including all dna fragments of the plurality of samples; then the plurality of samples are pooled to allow a single-tube reaction of the plurality of samples; and then the subsequent conversion, sequencing library construction and sequencing, distribution and decoding of readings of each sample, and downstream analysis are conducted. the library construction technology of the present application has advantages such as high efficiency, low cost, and stable and convenient operations.
