ETH ZÜRICH (20240209357). DNA CONSTRUCTS COMPRISING ALTERNATIVE PROMOTERS simplified abstract
Contents
DNA CONSTRUCTS COMPRISING ALTERNATIVE PROMOTERS
Organization Name
Inventor(s)
Yaakov Benenson of Binningen (CH)
DNA CONSTRUCTS COMPRISING ALTERNATIVE PROMOTERS - A simplified explanation of the abstract
This abstract first appeared for US patent application 20240209357 titled 'DNA CONSTRUCTS COMPRISING ALTERNATIVE PROMOTERS
The invention pertains to biomolecular computing and synthetic biology, specifically a DNA construct containing promoters and an output sequence to produce RNA in eukaryotic cells.
- DNA construct includes multiple promoters with transcription start sites for initiating transcription.
- Transcription from each promoter is enabled by specific transcriptional regulatory states.
- Output RNA is generated in eukaryotic cells when the regulatory states are present.
- The output RNA can be used for medical and diagnostic purposes in detecting, killing, or manipulating eukaryotic target cells.
Potential Applications: - Medical diagnostics for detecting specific eukaryotic cells. - Therapeutic applications for killing or manipulating target cells. - Research purposes in studying gene expression and regulation.
Problems Solved: - Enables precise control over gene expression in eukaryotic cells. - Facilitates targeted manipulation of specific cell types. - Provides a versatile tool for various biological applications.
Benefits: - Enhanced precision in gene expression control. - Potential for targeted therapies with minimal off-target effects. - Versatile tool for research and medical applications.
Commercial Applications: Title: "Innovative DNA Construct for Precision Gene Expression Control" This technology could be utilized in pharmaceutical research, biotechnology companies, and medical diagnostics firms for developing targeted therapies and diagnostic tools.
Questions about DNA Constructs: 1. How can this DNA construct be used in personalized medicine?
This DNA construct can potentially be tailored to target specific genetic markers in individual patients, allowing for personalized treatments.
2. What are the implications of using multiple promoters in a single DNA construct?
By incorporating multiple promoters, this technology offers greater flexibility and control over gene expression, enabling more precise manipulation of cellular processes.
Original Abstract Submitted
the invention relates to the fields of biomolecular computing and/or synthetic biology. in particular, the invention relates to a dna construct that comprises a first promoter, at least one further promoter and an output sequence, wherein each of said promoters comprises a transcription start site and/or is suitable for initiating transcription, wherein the initiation of transcription from the first promoter is enabled by a first transcriptional regulatory state and the initiation of transcription from each of said further promoter(s) is enabled by a respective further transcriptional regulatory state, and wherein said dna construct yields an effective amount of an output rna in a eukaryotic cell, when said first transcriptional regulatory state and/or any of the respective further transcriptional regulatory states is present in said cell, wherein said output rna comprises a sequence corresponding to said output sequence. furthermore, the invention relates to medical and/or diagnostic uses of the inventive dna construct of the invention, e.g., for detecting, killing and/or manipulating different types of eukaryotic target cells in a subject and/or in a tissue sample.
