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20250163443. Methods Pr (NORTH CAROLINA STATE UNIVERSITY)

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METHODS FOR PRODUCING LOW-NICOTINE TOBACCO

Abstract: the present technology provides methods of combining mutations in n-methylputrescine oxidase (mpo) gene isoforms with mutations in the three major berberine bridge enzyme-like (bbl) genes to produce low-nicotine tobacco plants and which prevent the accumulation of anatabine in the tobacco plants. also provided are methods of combining these mutations to produce tobacco plants, tobacco cells, and tobacco products having reduced nicotine content and which avoid the unwanted consequence of elevated anatabine levels in the tobacco plants, cells, and products.

Inventor(s): Ralph E. DEWEY, Ramsey S. LEWIS

CPC Classification: C12N15/8243 (for plant cells {, e.g. plant artificial chromosomes (PACs)})

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