19016805. SYSTEMS AND METHODS FOR CELLULAR ANALYSIS USING NUCLEIC ACID SEQUENCING (10x Genomics, Inc.)

SYSTEMS AND METHODS FOR CELLULAR ANALYSIS USING NUCLEIC ACID SEQUENCING

Organization Name

10x Genomics, Inc.

Inventor(s)

Xinying Zheng of San Jose CA US

SYSTEMS AND METHODS FOR CELLULAR ANALYSIS USING NUCLEIC ACID SEQUENCING

This abstract first appeared for US patent application 19016805 titled 'SYSTEMS AND METHODS FOR CELLULAR ANALYSIS USING NUCLEIC ACID SEQUENCING

Original Abstract Submitted

Template nucleic acid fragments are generated in cells or nuclei using transposase-nucleic acid complexes. Partitions are formed, each comprising a single cell or nuclei, the corresponding plurality of template nucleic acid fragments and nucleic acid barcodes comprising a corresponding common barcode sequence unique to a respective cell or nuclei. Barcoded nucleic acid fragments are generated in each partition using the barcodes and the template fragments. The barcoded fragments in each partition collectively form a pool of barcoded nucleic acid fragments. A set of alleles for each locus in a plurality of loci are identified and, for each such locus, a subset of the pool of barcoded fragments mapping to the locus are aligned to determine an allelic identity of such fragments from among the set of alleles for the locus, thereby determining a corresponding allelic distribution at each respective locus. These distributions are used to identify a structural variation.