18376018. METHODS AND KITS FOR USING RECOMBINANT MICROORGANISMS AS DIRECT REAGENTS IN BIOLOGICAL APPLICATIONS simplified abstract (Board of Regents, The University of Texas System)

METHODS AND KITS FOR USING RECOMBINANT MICROORGANISMS AS DIRECT REAGENTS IN BIOLOGICAL APPLICATIONS

Organization Name

Board of Regents, The University of Texas System

Inventor(s)

Andrew Ellington of Austin TX (US)

Sanchita Bhadra of Austin TX (US)

Jared Ellefson of Tucson AZ (US)

Jimmy Gollihar of Boston MA (US)

Arti Pothukuchy of Austin TX (US)

Michelle Byrom of Austin TX (US)

Raghav Shroff of Austin TX (US)

METHODS AND KITS FOR USING RECOMBINANT MICROORGANISMS AS DIRECT REAGENTS IN BIOLOGICAL APPLICATIONS - A simplified explanation of the abstract

This abstract first appeared for US patent application 18376018 titled 'METHODS AND KITS FOR USING RECOMBINANT MICROORGANISMS AS DIRECT REAGENTS IN BIOLOGICAL APPLICATIONS

Simplified Explanation

The abstract describes a method of utilizing an enzyme in a nucleic acid manipulation process without the need to purify the enzyme beforehand. This method involves transforming a microorganism with a non-native enzyme, inducing its expression, and adding the microorganism directly to the nucleic acid manipulation process.

• Simplified Explanation:

- Method of using an enzyme in nucleic acid manipulation without prior purification. - Transform microorganism with non-native enzyme, induce expression, and add directly to process. - Kit includes microorganism expressing enzyme, nucleic acids, and reagents for manipulation.

• • Potential Applications:

- Biotechnology research - Genetic engineering - Pharmaceutical development

• • Problems Solved:

- Eliminates the need for enzyme purification - Simplifies nucleic acid manipulation processes

• • Benefits:

- Cost-effective - Time-saving - Streamlines workflow

• • Potential Commercial Applications:

- Biotech companies - Pharmaceutical companies - Research institutions

• • Possible Prior Art:

- Traditional methods require enzyme purification before use in nucleic acid manipulation processes.

1. 1. 1. Unanswered Questions:

1. 1. 1. 1. 1. What specific non-native enzymes have been successfully utilized in this method?

- The abstract does not mention the specific enzymes used in the process.

1. 1. 1. 1. 2. Are there any limitations to the types of nucleic acid manipulation processes where this method can be applied?

- The abstract does not address any potential limitations in the application of this method to different nucleic acid manipulation processes.

Original Abstract Submitted

Disclosed herein is a method of utilizing an enzyme in a nucleic acid manipulation process, the method comprising: a) transforming a microorganism with a non-native enzyme; b) inducing expression of the enzyme in the microorganism, thereby producing the non-native enzyme; c) adding the microorganism of step b) directly to a non-naturally occurring nucleic acid manipulation process, wherein the non-native enzyme is not purified from the microorganism prior to addition to the nucleic acid manipulation process; and carrying out the nucleic acid manipulation process using the enzyme. Importantly, this method can be carried out without the need to purify the enzyme from the cell producing it before it is used in the nucleic acid manipulation method. Also disclosed herein is a kit for carrying out a nucleic acid manipulation process, the kit comprising a) a microorganism expressing a non-native enzyme; b) nucleic acids of interest; and c) reagents for use in the nucleic acid manipulation process.