18751730. Methods and Compositions for RNA-Directed Target DNA Modification and For RNA-Directed Modulation of Transcription (THE REGENTS OF THE UNIVERSITY OF CALIFORNIA)

Methods and Compositions for RNA-Directed Target DNA Modification and For RNA-Directed Modulation of Transcription

Organization Name

THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor(s)

Jennifer A. Doudna of Berkeley CA (US)

Martin Jinek of Berkeley CA (US)

Krzysztof Chylinski of Vienna (AT)

Emmanuelle Charpentier of Berlin (DE)

Methods and Compositions for RNA-Directed Target DNA Modification and For RNA-Directed Modulation of Transcription

This abstract first appeared for US patent application 18751730 titled 'Methods and Compositions for RNA-Directed Target DNA Modification and For RNA-Directed Modulation of Transcription

Original Abstract Submitted

The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.