Northwest university (20240401016). BETA-GLYCOSIDASE SS-BGL MUTANT FOR MODIFYING GINSENOSIDE AND APPLICATION THEREOF
BETA-GLYCOSIDASE SS-BGL MUTANT FOR MODIFYING GINSENOSIDE AND APPLICATION THEREOF
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BETA-GLYCOSIDASE SS-BGL MUTANT FOR MODIFYING GINSENOSIDE AND APPLICATION THEREOF
This abstract first appeared for US patent application 20240401016 titled 'BETA-GLYCOSIDASE SS-BGL MUTANT FOR MODIFYING GINSENOSIDE AND APPLICATION THEREOF
Original Abstract Submitted
a �-glycosidase ss-bgl mutant for modifying ginsenoside and application thereof are provided, which relate to the field of genetic engineering technologies. the �-glycosidase ss-bgl mutant is �-glycosidase ss-bgl with the amino acid sequence as shown in seq id no: 1 having mutation at one or more sites. a single-site mutation includes: a1, making glutamine at 96position of seq id no: 1 to be mutated to glutamic acid; a2, making asparagine at 97position of seq id no: 1 to be mutated to aspartate; a3, making asparagine at 128position of seq id no: 1 to be mutated to aspartate; a4, making asparagine at 302position of seq id no: 1 to be mutated to aspartate. a double-site mutation is a combination of a3 and a4, a three-site mutation is a combination of a1, a2 and a4, and a four-site mutation is a combination of a1, a2, a3 and a4.
