Google llc (20240344112). ITERATIVE OLIGONUCLEOTIDE BARCODE EXPANSION FOR LABELING AND LOCALIZING MANY BIOMOLECULES simplified abstract
Contents
ITERATIVE OLIGONUCLEOTIDE BARCODE EXPANSION FOR LABELING AND LOCALIZING MANY BIOMOLECULES
Organization Name
Inventor(s)
Ali Bashir of San Mateo CA (US)
Marc Berndl of Mountain View CA (US)
Annalisa Pawlosky of Mountain View CA (US)
Jun Kim of Mountain View CA (US)
Sara Ahadi of Mountain View CA (US)
Alexander Tran of Mountain View CA (US)
ITERATIVE OLIGONUCLEOTIDE BARCODE EXPANSION FOR LABELING AND LOCALIZING MANY BIOMOLECULES - A simplified explanation of the abstract
This abstract first appeared for US patent application 20240344112 titled 'ITERATIVE OLIGONUCLEOTIDE BARCODE EXPANSION FOR LABELING AND LOCALIZING MANY BIOMOLECULES
The abstract of this patent application describes a method for improving gene sequencing techniques by inserting dual polynucleotide 'bar-codes' into a target polynucleotide, allowing for more accurate and cost-effective alignment of read fragments.
- Dual polynucleotide 'bar-codes' are inserted into a target polynucleotide and mechanically connected via a linker.
- The barcodes can be 'grown' via a pool-split-pool process, linking polynucleotide fragments with the same complete barcode sequence.
- Joined fragments can be separated and sequenced, with each read sequence beginning with a regionally-specific barcode for accurate association of fragments from the region.
- This method increases accuracy and reduces computational cost in aligning read fragments and performing other sequencing processes.
Potential Applications: - Improving gene sequencing accuracy and efficiency - Enhancing research in genetics and genomics - Facilitating personalized medicine and diagnostics
Problems Solved: - Difficulty and expense in aligning generated read fragments - Inaccuracies in sequencing due to limitations of existing techniques
Benefits: - Increased accuracy in gene sequencing - Cost-effective alignment of read fragments - Enhanced research capabilities in genetics and genomics
Commercial Applications: Title: Enhanced Gene Sequencing Technology for Precision Medicine and Research This technology can be utilized in research institutions, biotechnology companies, and healthcare settings for gene sequencing, personalized medicine, and genetic diagnostics.
Questions about the technology: 1. How does this method improve the accuracy of gene sequencing? 2. What are the potential cost savings associated with using this technology in research and healthcare settings?
Frequently Updated Research: Stay updated on advancements in gene sequencing techniques, personalized medicine applications, and genetic diagnostics to enhance the use of this technology in various fields.
Original Abstract Submitted
contemporary gene sequencing techniques, including “next. generation sequencing” techniques, can include sequencing a plurality of fragments of a target polynucleotide. however, the limitations of existing sequencing techniques means that it can be difficult and/or expensive to align the generated read fragments. methods provided herein include inserting dual polynucleotide ‘bar-codes’ into a target poly nucleotide that remain mechanically connected via a tinker.’ tire barcodes can then be ‘grown’ via. a. pool-split-pool process such that polynucleotide fragments that are linked by linkers exhibit the same complete barcode sequence that is different, from the complete barcode sequence exhibited by non-linked polynucleotide fragments. the joined fragments can then be separated and sequenced. each read sequence thus begins with a regionally-specific barcode that can be used to associate fragments from the region together, allowing for increased accuracy and reduced computational cost in aligning the read fragments and/or performing other sequencing processes on the read fragments.
