President and Fellows of Harvard College (20240327901). Methods of Generating Libraries of Nucleic Acid Sequences for Detection via Flourescent in Situ Sequ simplified abstract

Methods of Generating Libraries of Nucleic Acid Sequences for Detection via Flourescent in Situ Sequ

Organization Name

President and Fellows of Harvard College

Inventor(s)

George M. Church of Brookline MA (US)

Evan R. Daugharthy of Cambridge MA (US)

Richard C. Terry of Carlisle MA (US)

Benjamin W. Pruitt of Cambridge MA (US)

Brian M. Turczyk of Westford MA (US)

Methods of Generating Libraries of Nucleic Acid Sequences for Detection via Flourescent in Situ Sequ - A simplified explanation of the abstract

This abstract first appeared for US patent application 20240327901 titled 'Methods of Generating Libraries of Nucleic Acid Sequences for Detection via Flourescent in Situ Sequ

The present disclosure introduces targeted nucleic acid FISSEQ library construction methods that offer benefits such as enhanced sensitivity and shorter assay times for detecting, identifying, quantifying, and determining the nucleotide sequence of target species compared to random or whole-omic detection via FISSEQ.

• Targeted FISSEQ library construction methods
• Enhanced sensitivity in detection
• Shorter assay times
• Improved identification, quantification, and nucleotide sequencing of target species
• Contrasted with random or whole-omic detection via FISSEQ

Potential Applications: - Biomedical research - Clinical diagnostics - Environmental monitoring

Problems Solved: - Increased sensitivity in nucleic acid detection - Reduced assay times for analysis - Enhanced accuracy in identifying target species

Benefits: - Improved efficiency in nucleic acid analysis - Enhanced accuracy in target species identification - Faster results in detection and sequencing

Commercial Applications: Title: Enhanced Nucleic Acid Analysis Methods for Biomedical and Environmental Applications This technology can be utilized in research laboratories, diagnostic centers, and environmental monitoring agencies to improve nucleic acid analysis processes, leading to more accurate and efficient results.

Questions about the technology: 1. How does targeted FISSEQ library construction improve sensitivity in nucleic acid detection? 2. What are the specific benefits of using targeted FISSEQ methods in clinical diagnostics?

Frequently Updated Research: Researchers are continuously exploring new applications and optimizations of targeted FISSEQ library construction methods in various fields, including genomics, microbiology, and environmental science. Stay updated on the latest advancements in this technology to leverage its full potential.

Original Abstract Submitted

the present disclosure provides a number of targeted nucleic acid fisseq library construction methods. targeted fisseq can exhibit several benefits, such as enhanced sensitivity and/or shorter assay time in the detection, identification, quantification, and/or determining the nucleotide sequence of the target species, relative to “random” or “whole-omic” detection via fisseq.