View source for TOPPAN HOLDINGS INC. (20250115949). METHODS FOR DETECTING FLUORESCENCE

=METHODS FOR DETECTING FLUORESCENCE=


==Organization Name==

[[:Category:TOPPAN HOLDINGS INC.|TOPPAN HOLDINGS INC.]]


[[Category:TOPPAN HOLDINGS INC.]]

==Inventor(s)==

[[:Category:Masayuki Ogino of Taito-ku JP|Masayuki Ogino of Taito-ku JP]][[Category:Masayuki Ogino of Taito-ku JP]]

[[:Category:Misaki Nagahara of Taito-ku JP|Misaki Nagahara of Taito-ku JP]][[Category:Misaki Nagahara of Taito-ku JP]]

[[:Category:Yoichi Makino of Taito-ku JP|Yoichi Makino of Taito-ku JP]][[Category:Yoichi Makino of Taito-ku JP]]

[[:Category:Yosuke Horiuchi of Taito-ku JP|Yosuke Horiuchi of Taito-ku JP]][[Category:Yosuke Horiuchi of Taito-ku JP]]

[[:Category:Yuta Suzuki of Taito-ku JP|Yuta Suzuki of Taito-ku JP]][[Category:Yuta Suzuki of Taito-ku JP]]

==METHODS FOR DETECTING FLUORESCENCE==


This abstract first appeared for US patent application 20250115949 titled 'METHODS FOR DETECTING FLUORESCENCE

==Original Abstract Submitted==

a single-stranded oligonucleotide labeled with a fluorescent substance and a quenching substance. in the single-stranded oligonucleotide, a hairpin structure is formed by self-hybridization on the 5′ side, and when a single-stranded oligonucleotide having a complementary base sequence on the 3′ side hybridizes, a triplex structure is formed on the 5′ end portion, and cleaved by a flap endonuclease that recognizes the triplex structure, whereby the fluorescent substance is released from the quenching substance, the fluorescent substance emitting fluorescence when irradiated with excitation light, and the single-stranded nucleotide is a single-stranded oligonucleotide including a non-complementary base pair within the hairpin structure, a single-stranded oligonucleotide in which a nucleotide residue labeled with the fluorescent substance and a nucleotide residue labeled with the quenching substance are separated from each other by 4 or more residues, or a single-stranded oligonucleotide in which the fluorescent substance is bound to a portion other than the base.



[[Category:C12Q1/6818]]
[[Category:G01N21/64]]
[[Category:CPC_C12Q1/6818]]