View source for Northwest university (20240318153). Construction Method and Applications of Glycosyltransferase BS-YjiC Mutant simplified abstract

=Construction Method and Applications of Glycosyltransferase BS-YjiC Mutant=


==Organization Name==

[[:Category:Northwest university|Northwest university]]


[[Category:Northwest university]]

==Inventor(s)==

[[:Category:Weina Li of Xi’an (CN)|Weina Li of Xi’an (CN)]][[Category:Weina Li of Xi’an (CN)]]

[[:Category:Daidi Fan of Xi’an (CN)|Daidi Fan of Xi’an (CN)]][[Category:Daidi Fan of Xi’an (CN)]]

[[:Category:Chenhui Zhu of Xi’an (CN)|Chenhui Zhu of Xi’an (CN)]][[Category:Chenhui Zhu of Xi’an (CN)]]

==Construction Method and Applications of Glycosyltransferase BS-YjiC Mutant - A simplified explanation of the abstract==


This abstract first appeared for US patent application 20240318153 titled 'Construction Method and Applications of Glycosyltransferase BS-YjiC Mutant

'''Simplified Explanation:'''
The patent application relates to a glycosyltransferase bs-yjic mutant obtained through site-directed mutation, which is more effective in catalyzing protopanaxadiol to produce rare ginsenosides F12 and Rh2.

* The glycosyltransferase bs-yjic mutant is created by mutating specific amino acids in the wild-type glycosyltransferase bs-yjic.
* The mutant enzyme is better suited for converting protopanaxadiol into rare ginsenosides F12 and Rh2.
* This innovation enhances the flexibility of the production process for these valuable compounds.

'''Potential Applications:'''
1. Pharmaceutical industry for the production of rare ginsenosides.
2. Biotechnology companies for enzyme engineering applications.
3. Research institutions for studying glycosylation processes.

'''Problems Solved:'''
1. Enhancing the efficiency of converting protopanaxadiol to rare ginsenosides.
2. Improving the specificity of the glycosyltransferase enzyme.
3. Streamlining the production process of valuable compounds.

'''Benefits:'''
1. Increased yield of rare ginsenosides.
2. Enhanced enzymatic activity for specific glycosylation reactions.
3. Simplified and optimized production processes.

'''Commercial Applications:'''
Optimizing Enzyme Engineering for Rare Ginsenoside Production

'''Questions about Glycosyltransferase bs-yjic Mutant:'''
1. How does the glycosyltransferase bs-yjic mutant improve the production of rare ginsenosides?
2. What are the potential implications of this innovation in the pharmaceutical industry?

'''Frequently Updated Research:'''
Stay updated on the latest advancements in enzyme engineering for glycosylation processes to improve rare ginsenoside production.


==Original Abstract Submitted==

a glycosyltransferase bs-yjic mutant, a construction method and an application thereof provided, relating to the field of genetic engineering technologies. the glycosyltransferase bs-yjic mutant is obtained by performing site-directed mutation on amino acids at the 125, 178, 313, 125and 178, or 125and 313positions of a wild-type glycosyltransferase bs-yjic with the amino acid sequence as shown in seq id no: 1. compared with the wild-type glycosyltransferase bs-yjic, the glycosyltransferase bs-yjic mutant is more suitable for catalyzing protopanaxadiol (ppd) to generate rare ginsenosides f12 and rh2, which is more conducive to the flexibility of the production process.



[[Category:C12N9/10]]
[[Category:C12N15/70]]
[[Category:C12P17/06]]
[[Category:CPC_C12N9/1051]]