View source for 20250231189. Measuring Frequenc (Thomas Jefferson University)

= MEASURING FREQUENCY OF PATHOGEN-SPECIFIC T CELLS IN PERIPHERAL BLOOD AS ESTABLISHED BY TCR-INDUCED CA(2+) SIGNALING =

'''Abstract''':
a method for measuring kinetics of caflux in differentially responding t cells that form monolayer on the glass surface in response to antigenic peptides or live target cells comprising: immobilizing t cells labeled with casensitive fluorophore on the glass bottom of a well, covered with capturing antibody or a capturing protein that bind to non-stimulatory t-cell surface receptor; adding to the well a single or multiple peptide epitopes that binds to the cell surface mhc molecules to be presented for recognition by cognate t cells; the stimulatory signal could also be delivered by live target cells that display peptide epitope(s); wherein the recognition of stimulatory of pmhc by the peptide specific t cells leads to increase of intracellular calevel and fluorescence intensity in the responding t cells, which is then identified after the subtracting fluorescence intensity for every t cell before and after the addition of the peptide antigens; scoring each responding t cell into a category according to three categories including: a rapid and sustained t-cell response, an oscillatory response, or a delayed and oscillatory response; and measuring changes in number of an individual t cells with increased intracellular fluorescence as function of time provides the kinetic curve of the tcr-mediated ca signaling.

'''Inventor(s)''': [[:Category:Yuri Sykulev of Newtown Square PA US|Yuri Sykulev]], [[:Category:Nadezhda Anikeyeva of Bryn Mawr PA US|Nadezhda Anikeyeva]]

'''CPC Classification''': [[:Category:CPC_G01N33/582|G01N33/582]] ({with fluorescent label})


[[Category:Patent Applications]]
[[Category:Thomas Jefferson University]]
[[Category:CPC_G01N33/582]]
[[Category:Yuri Sykulev of Newtown Square PA US]]
[[Category:Nadezhda Anikeyeva of Bryn Mawr PA US]]

[https://wikipatents.org/index.php?search=20250231189+rejection&title=Special%3ASearch&wprov=acrw1_-1 Search for rejections for patent application number 20250231189]


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