View source for 18472881. RATIONALLY-DESIGNED MEGANUCLEASES WITH ALTERED SEQUENCE SPECIFICITY AND DNA-BINDING AFFINITY simplified abstract (Duke University)

=RATIONALLY-DESIGNED MEGANUCLEASES WITH ALTERED SEQUENCE SPECIFICITY AND DNA-BINDING AFFINITY=


==Organization Name==

[[:Category:Duke University|Duke University]]


[[Category:Duke University]]

==Inventor(s)==

[[:Category:James Jefferson Smith of Morrisville NC (US)|James Jefferson Smith of Morrisville NC (US)]][[Category:James Jefferson Smith of Morrisville NC (US)]]

[[:Category:Derek Jantz of Durham NC (US)|Derek Jantz of Durham NC (US)]][[Category:Derek Jantz of Durham NC (US)]]

[[:Category:Homme W. Hellinga of Durham NC (US)|Homme W. Hellinga of Durham NC (US)]][[Category:Homme W. Hellinga of Durham NC (US)]]

==RATIONALLY-DESIGNED MEGANUCLEASES WITH ALTERED SEQUENCE SPECIFICITY AND DNA-BINDING AFFINITY - A simplified explanation of the abstract==


This abstract first appeared for US patent application 18472881 titled 'RATIONALLY-DESIGNED MEGANUCLEASES WITH ALTERED SEQUENCE SPECIFICITY AND DNA-BINDING AFFINITY

Abstract: Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.

*Simplified Explanation:
This patent application discusses the creation of rationally-designed LAGLIDADG meganucleases and their use in inserting specific DNA sequences into limited loci in the genome for various applications.

*Key Features and Innovation:
- Development of rationally-designed LAGLIDADG meganucleases
- Insertion of desired DNA sequences into specific loci in the genome
- Applications in gene therapy, treatment of pathogenic infections, diagnostics, and research

*Potential Applications:
- Gene therapy
- Treatment of pathogenic infections
- Diagnostic applications
- Research purposes

*Problems Solved:
- Precise insertion of DNA sequences into the genome
- Limited number of loci targeted for insertion
- Improved gene therapy techniques

*Benefits:
- Enhanced precision in genetic modifications
- Targeted insertion of DNA sequences
- Potential for more effective gene therapy treatments

*Commercial Applications:
Potential commercial applications include genetic engineering services, biotechnology research tools, and development of novel gene therapy treatments.

*Questions about LAGLIDADG Meganucleases:
1. How do rationally-designed LAGLIDADG meganucleases differ from other gene editing tools?
2. What are the specific challenges associated with using meganucleases for gene therapy applications?


==Original Abstract Submitted==

Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.



[[Category:C12N9/22]]
[[Category:A61K38/46]]
[[Category:C12N15/82]]
[[Category:C12N15/90]]
[[Category:CPC_C12N9/22]]