View source for 18455148. IMAGING FLOW CYTOMETER USING SPATIAL-TEMPORAL TRANSFORMATION simplified abstract (THE REGENTS OF THE UNIVERSITY OF CALIFORNIA)

=IMAGING FLOW CYTOMETER USING SPATIAL-TEMPORAL TRANSFORMATION=


==Organization Name==

[[:Category:THE REGENTS OF THE UNIVERSITY OF CALIFORNIA|THE REGENTS OF THE UNIVERSITY OF CALIFORNIA]]


[[Category:THE REGENTS OF THE UNIVERSITY OF CALIFORNIA]]

==Inventor(s)==

[[:Category:Yu-Hwa Lo of San Diego CA (US)|Yu-Hwa Lo of San Diego CA (US)]][[Category:Yu-Hwa Lo of San Diego CA (US)]]

[[:Category:Yuanyuan Han of San Diego CA (US)|Yuanyuan Han of San Diego CA (US)]][[Category:Yuanyuan Han of San Diego CA (US)]]

==IMAGING FLOW CYTOMETER USING SPATIAL-TEMPORAL TRANSFORMATION - A simplified explanation of the abstract==


This abstract first appeared for US patent application 18455148 titled 'IMAGING FLOW CYTOMETER USING SPATIAL-TEMPORAL TRANSFORMATION

==Simplified Explanation==

The patent application describes a method for imaging particles and/or cells using flow cytometry, involving transmitting a light beam at a fluidic channel carrying a fluid sample containing particles, optically encoding scattered or fluorescently-emitted light at a spatial optical filter, and producing image data associated with the particle flow based on the encoded optical signal.

* Method for imaging particles and/or cells using flow cytometry
* Transmission of a light beam at a fluidic channel carrying a fluid sample containing particles
* Optical encoding of scattered or fluorescently-emitted light at a spatial optical filter
* Producing image data associated with the particle flow based on the encoded optical signal
* Image data includes information of a physical characteristic of the particle

== Potential Applications ==
The technology can be applied in medical diagnostics, biological research, pharmaceutical development, and environmental monitoring.

== Problems Solved ==
This technology allows for high-throughput imaging of particles and cells in a fluid sample, providing valuable data for various scientific and medical applications.

== Benefits ==
The method offers a rapid and efficient way to analyze and characterize particles and cells in a fluid sample, enabling researchers and clinicians to gain insights into cellular properties and functions.

== Potential Commercial Applications ==
The technology could be utilized in clinical laboratories, research institutions, biotechnology companies, and pharmaceutical companies for various applications such as disease diagnosis, drug discovery, and environmental testing.

== Possible Prior Art ==
Prior art in flow cytometry imaging techniques may include methods using traditional optical filters and detectors to analyze scattered or fluorescent light from particles in a fluid sample.

=== Unanswered Questions ===

=== How does this technology compare to existing flow cytometry imaging methods? ===

This technology offers a unique approach to imaging particles and cells in a fluid sample by using a spatial optical filter to encode scattered or fluorescent light. It would be interesting to know how this method compares in terms of sensitivity, resolution, and throughput with traditional flow cytometry techniques.

=== What are the potential limitations or challenges of implementing this technology in real-world applications? ===

While the method described in the patent application shows promise for imaging particles and cells in a fluid sample, there may be challenges in scaling up the technology for high-throughput analysis, optimizing image processing algorithms, and integrating the system into existing flow cytometry platforms. Further research and development may be needed to address these potential limitations.


==Original Abstract Submitted==

Methods, systems, and devices are disclosed for imaging particles and/or cells using flow cytometry. In one aspect, a method includes transmitting a light beam at a fluidic channel carrying a fluid sample containing particles; optically encoding scattered or fluorescently-emitted light at a spatial optical filter, the spatial optical filter including a surface having a plurality of apertures arranged in a pattern along a transverse direction opposite to particle flow and a longitudinal direction parallel to particle flow, such that different portions of a particle flowing over the pattern of the apertures pass different apertures at different times and scatter the light beam or emit fluorescent light at locations associated with the apertures; and producing image data associated with the particle flowing through the fluidic channel based on the encoded optical signal, in which the produced image data includes information of a physical characteristic of the particle.



[[Category:G01N21/64]]
[[Category:G01N15/14]]
[[Category:G01N21/51]]
[[Category:G01N21/53]]