US Patent Application 18219079. UNIVERSAL TEMPLATE STRANDS FOR ENZYMATIC POLYNUCLEOTIDE SYNTHESIS simplified abstract
Contents
UNIVERSAL TEMPLATE STRANDS FOR ENZYMATIC POLYNUCLEOTIDE SYNTHESIS
Organization Name
MICROSOFT TECHNOLOGY LICENSING, LLC
Inventor(s)
Bichlien Nguyen of Seattle WA (US)
Robert Carlson of Seattle WA (US)
Karin Strauss of Seattle WA (US)
UNIVERSAL TEMPLATE STRANDS FOR ENZYMATIC POLYNUCLEOTIDE SYNTHESIS - A simplified explanation of the abstract
This abstract first appeared for US patent application 18219079 titled 'UNIVERSAL TEMPLATE STRANDS FOR ENZYMATIC POLYNUCLEOTIDE SYNTHESIS
Simplified Explanation
- The patent application describes a method for synthesizing polynucleotides using a universal template strand. - The universal template strand is made up of universal base analogs and can hybridize to any sequence of nucleotides. - A primer is used to initiate the synthesis of a new polynucleotide on the universal template strand. - The sequence of the new polynucleotide is determined by the order of addition of protected nucleotides, rather than by base pairing with the template strand. - After each addition of a protected nucleotide, the blocking group is removed and another protected nucleotide is added. - The order of nucleotide addition can be varied to create any desired sequence. - Once synthesis is complete, the polynucleotide can be separated from the universal template strand. - The universal template strand can then be reused to synthesize a different polynucleotide.
Original Abstract Submitted
A universal template strand built with universal base analogs is used as a template for polynucleotide synthesis. The universal template strand can hybridize to any sequence of nucleotides. A new polynucleotide is synthesized by using a polymerase to extend a primer hybridized to the universal template strand. Unlike primer extension in polymerase chain reactions, base pairing with nucleotides in the template strand does not specify the sequence of the new polynucleotide. Instead, the sequence of the new polynucleotide is specified by the order of addition of protected nucleotides. After addition of a single species of protected nucleotide, the blocking group is removed and another protected nucleotide is added. The order of nucleotide addition can be varied to create any sequence. After synthesis, the polynucleotide can be dehybridized from the universal template strand. The universal template strand may then be reused to synthesize a different polynucleotide.
