US Patent Application 17721777. NON-AMPLIFIABLE POLYNUCLEOTIDES FOR ENCODING INFORMATION simplified abstract
Contents
NON-AMPLIFIABLE POLYNUCLEOTIDES FOR ENCODING INFORMATION
Organization Name
Microsoft Technology Licensing, LLC
Inventor(s)
Bichlien Hoang Nguyen of Seattle WA (US)
Yuan-Jyue Chen of Seattle WA (US)
Jake Allen Smith of Seattle WA (US)
NON-AMPLIFIABLE POLYNUCLEOTIDES FOR ENCODING INFORMATION - A simplified explanation of the abstract
- This abstract for appeared for US patent application number 17721777 Titled 'NON-AMPLIFIABLE POLYNUCLEOTIDES FOR ENCODING INFORMATION'
Simplified Explanation
The abstract discusses the use of special molecules called polynucleotides to encode information. These polynucleotides are made with base analogs that can interact with other bases but cannot form certain types of bonds. This prevents them from being amplified using a common technique called PCR. However, they can still bind to complementary strands and their sequences can be read using nanopore sequencing. These polynucleotides can be used as labels to prevent forgery or to encode digital data that cannot be easily copied. The data can be retrieved using nanopore sequencing and decoding the nucleotide sequence.
Original Abstract Submitted
Polynucleotides used for encoding information are synthesized with universal base analogs that participate in pi-stacking interactions but do not form Watson-Crick hydrogen bonds with other bases. The universal base analogs may have pyrrole-based bases such as 5-nitroindole (5NI). Inclusion of the universal base analogs in the polynucleotides prevents polymerase-based amplification such as PCR. However, the non-amplifiable polynucleotides are able to hybridize to complementary strands and the sequences may be read by nanopore sequencing. The polynucleotides may be used as molecular taggants to label items for the prevention of forgery. The ability of polynucleotides collected from an item to hybridize with known sequences can be used to establish authenticity of an item. Alternatively, the polynucleotides may be used to encode digital data in a read-only molecule that cannot be readily copied. The digital data can be retrieved from the polynucleotides by nanopore sequencing and decoding of the nucleotide sequence data.
