20240043798. METHODS AND COMPOSITIONS FOR GENERATING OLIGODENDROCYTE PROGENITOR CELLS simplified abstract (Trailhead Biosystems Inc.)

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METHODS AND COMPOSITIONS FOR GENERATING OLIGODENDROCYTE PROGENITOR CELLS

Organization Name

Trailhead Biosystems Inc.

Inventor(s)

Nooshin Amini of Cleveland OH (US)

METHODS AND COMPOSITIONS FOR GENERATING OLIGODENDROCYTE PROGENITOR CELLS - A simplified explanation of the abstract

This abstract first appeared for US patent application 20240043798 titled 'METHODS AND COMPOSITIONS FOR GENERATING OLIGODENDROCYTE PROGENITOR CELLS

Simplified Explanation

Methods for generating pre-oligodendrocyte progenitor cells (pre-OPCs), oligodendrocyte progenitor cells (OPCs), and pre-myelinating oligodendrocytes (preOLs) from human pluripotent stem cells are provided using chemically-defined culture media. These methods allow for the generation of pre-OPCs in as little as three days, Sox10+Olig2+Nkx2-2+OPCs in as little as twelve days, and CD9+A2B5+O4+CNPase+preOLs in as little as eighteen days. Two alternative culture protocols for generating OPCs are also provided. Additionally, culture media, isolated cell populations, and kits are provided.

  • Methods for generating pre-OPCs, OPCs, and preOLs from human pluripotent stem cells
  • Use of chemically-defined culture media
  • Rapid generation of pre-OPCs in three days, Sox10+Olig2+Nkx2-2+OPCs in twelve days, and CD9+A2B5+O4+CNPase+preOLs in eighteen days
  • Provision of two alternative culture protocols for generating OPCs
  • Provision of culture media, isolated cell populations, and kits

Potential applications of this technology:

  • Treatment of demyelinating diseases such as multiple sclerosis
  • Development of in vitro models for studying oligodendrocyte development and myelination
  • Drug discovery and screening for therapies targeting oligodendrocyte-related disorders

Problems solved by this technology:

  • Limited availability of pre-OPCs, OPCs, and preOLs for research and therapeutic purposes
  • Time-consuming and complex methods for generating these cell types
  • Lack of standardized culture media and protocols for efficient generation of pre-OPCs and OPCs

Benefits of this technology:

  • Rapid and efficient generation of pre-OPCs, OPCs, and preOLs from human pluripotent stem cells
  • Use of chemically-defined culture media eliminates the need for animal-derived components and enhances reproducibility
  • Provision of alternative culture protocols allows flexibility in experimental design
  • Availability of culture media, isolated cell populations, and kits facilitates widespread adoption and application of the technology.


Original Abstract Submitted

methods for generating pre-oligodendrocyte progenitor cells (pre-opcs), oligodendrocyte progenitor cells (opcs) and pre-myelinating oligodendrocytes (preols) from human pluripotent stem cells are provided using chemically-defined culture media that allow for generation of pre-opcs in as little as three days, sox10+olig2+nkx2-2+opcs in as little as twelve days and cd9+a2b5+o4+cnpase+preols in as little as eighteen days. two alternative culture protocols for generating opcs are provided. culture media, isolated cell populations and kits are also provided.

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