20240034991. METHOD FOR EFFICIENTLY INDUCING REPROGRAMMING OF HUMAN CELL INTO NEURONAL CELL simplified abstract (GUANGXI UNIVERSITY)
METHOD FOR EFFICIENTLY INDUCING REPROGRAMMING OF HUMAN CELL INTO NEURONAL CELL
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METHOD FOR EFFICIENTLY INDUCING REPROGRAMMING OF HUMAN CELL INTO NEURONAL CELL - A simplified explanation of the abstract
This abstract first appeared for US patent application 20240034991 titled 'METHOD FOR EFFICIENTLY INDUCING REPROGRAMMING OF HUMAN CELL INTO NEURONAL CELL
Simplified Explanation
The abstract describes a method for efficiently converting human cells into neuronal cells. This is achieved by increasing the concentration of cyclic adenosine monophosphate (cAMP) or up-regulating the expression of protein kinase A (PKA) and cAMP response element-binding protein (CREB), or inhibiting the expression of AMP-activated protein kinase (AMPK), ALK2, ALK3, p38, and JNK using a single small molecule compound or gene knockout or gene overexpression. The method is safe, quick, and highly efficient, with specific induction sites and genes, and a clear molecular regulation mechanism. The small molecule compound can be used in the clinical treatment of neurodegenerative diseases, providing a safer and more effective treatment method. Additionally, the method allows for the continuous production of induced neuronal cells from fibroblasts and astrocytes, which can divide and proliferate in vitro and in vivo.
- Efficient method for converting human cells into neuronal cells
- Increase cAMP concentration or up-regulate PKA and CREB expression
- Inhibit AMPK, ALK2, ALK3, p38, and JNK expression
- Achieved using a single small molecule compound or gene manipulation
- Safe, quick, and highly efficient induction process
- Specific induction sites and genes
- Clear molecular regulation mechanism
- Suitable for clinical treatment of neurodegenerative diseases
- Provides a safer and more effective treatment method
- Allows for continuous production of induced neuronal cells
- Fibroblasts and astrocytes can divide and proliferate in vitro and in vivo
Potential Applications
- Clinical treatment of neurodegenerative diseases
- Production of induced neuronal cells for research and drug discovery purposes
Problems Solved
- Lack of efficient methods for inducing reprogramming of human cells into neuronal cells
- Limited availability of induced neuronal cells for research and therapeutic purposes
Benefits
- Safe and quick induction process
- High efficiency in converting human cells into neuronal cells
- Specific induction sites and genes for targeted reprogramming
- Clear understanding of the molecular regulation mechanism
- Continuous production of induced neuronal cells from dividing and proliferating cells
Original Abstract Submitted
in a method for efficiently inducing reprogramming of human cells into neuronal cells, a concentration of camp is increased or an expression of pka and creb is up-regulated or an expression of ampk, alk2, alk3, p38, and jnk is inhibited by a single small molecule compound or gene knockout or gene overexpression. in the present disclosure, the induction small molecule compound is single and safe, and has a short induction time, high induction efficiency, definite induction sites and genes, and clear molecular regulation mechanism. the small molecule compound can be applied to the clinical treatment of human neurodegenerative diseases, providing a safer and more efficient treatment method for the neurodegenerative diseases. since neuronal cells cannot divide and proliferate, the method induces fibroblasts and astrocytes that can divide and proliferate in vitro and in vivo, to continuously obtain a large number of induced neuronal cells in vitro and in vivo.
