20240026338. METHOD AND APPARATUS FOR ENCODING CELLULAR SPATIAL POSITION INFORMATION simplified abstract (SILICON VALLEY SCIENTIFIC, INC.)

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METHOD AND APPARATUS FOR ENCODING CELLULAR SPATIAL POSITION INFORMATION

Organization Name

SILICON VALLEY SCIENTIFIC, INC.

Inventor(s)

Stevan Bogdan Jovanovich of Livermore CA (US)

Peter Wagner of Menlo Park CA (US)

METHOD AND APPARATUS FOR ENCODING CELLULAR SPATIAL POSITION INFORMATION - A simplified explanation of the abstract

This abstract first appeared for US patent application 20240026338 titled 'METHOD AND APPARATUS FOR ENCODING CELLULAR SPATIAL POSITION INFORMATION

Simplified Explanation

The patent application describes a system and methods for collecting and preparing single cells and groups of cells from microsamples of specimens. It also encodes spatial information of the physical position of the cells in the specimen.

  • The system uses beads or surfaces with oligonucleotides containing spatial barcodes to analyze DNA or RNA.
  • The spatial barcodes allow the position of the cell to be defined and the nucleic acid sequencing information to be analyzed in the context of the cell's physical position in the specimen.
  • Markers such as isotopes can also be added to a microsample to encode spatial position with mass spectrometry or other analysis methods.
  • The spatial encoded information can then be readout by analysis methods such as DNA sequencing, mass spectrometry, fluorescence, or other methods.

Potential applications of this technology:

  • Single-cell analysis for understanding cell types, gene expression patterns, and DNA sequences in a spatial context.
  • Spatially resolved transcriptomics and genomics studies.
  • Identification and characterization of rare cells or cell populations within a specimen.
  • Spatially mapping the distribution of specific genes or biomarkers within a tissue sample.

Problems solved by this technology:

  • Overcoming the limitations of traditional bulk analysis methods by enabling analysis at the single-cell level.
  • Providing spatial information to understand the organization and interactions of cells within a tissue sample.
  • Improving the accuracy and efficiency of analyzing DNA or RNA from microsamples.

Benefits of this technology:

  • Enables a deeper understanding of cellular heterogeneity and spatial organization within tissues.
  • Facilitates the identification of novel cell types or subpopulations.
  • Provides valuable insights into disease mechanisms, developmental processes, and tissue function.
  • Offers potential applications in fields such as cancer research, neuroscience, developmental biology, and regenerative medicine.


Original Abstract Submitted

a system, methods, and apparatus are described to collect and prepare single cells and groups of cells from microsamples of specimens and encode spatial information of the physical position of the cells in the specimen. in some embodiment, beads or surfaces with oligonucleotides containing spatial barcodes are used to analyze dna or rna. the spatial barcodes allow the position of the cell to be defined and the nucleic acid sequencing information, such as target sequencing, whole genome, gene expression, used to analyze the cells in a microsample for cell type, expression pattern, dna sequence, and other information, in the context of the cell's physical position in the specimen. in other embodiment, markers such as isotopes are added to a microsample to encode spatial position with mass spectoscopy or other analysis. the spatial encoded information is then readout by analysis such as dna sequencing, mass spectrometry, fluorescence, or other methods.

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