20240019365. METHOD FOR ANALYSING A BIOLOGICAL SAMPLE COMPRISING DETERMINATION OF THE SPATIAL DISTRIBUTION OF BIOMASS ALONG THE OPTICAL AXIS simplified abstract (BIOMERIEUX)

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METHOD FOR ANALYSING A BIOLOGICAL SAMPLE COMPRISING DETERMINATION OF THE SPATIAL DISTRIBUTION OF BIOMASS ALONG THE OPTICAL AXIS

Organization Name

BIOMERIEUX

Inventor(s)

Dominique Decaux of Chaponost (FR)

Emilie Bisceglia of La Tour de Salvagny (FR)

METHOD FOR ANALYSING A BIOLOGICAL SAMPLE COMPRISING DETERMINATION OF THE SPATIAL DISTRIBUTION OF BIOMASS ALONG THE OPTICAL AXIS - A simplified explanation of the abstract

This abstract first appeared for US patent application 20240019365 titled 'METHOD FOR ANALYSING A BIOLOGICAL SAMPLE COMPRISING DETERMINATION OF THE SPATIAL DISTRIBUTION OF BIOMASS ALONG THE OPTICAL AXIS

Simplified Explanation

The patent application describes a method for analyzing a biological sample using holographic imaging. The method involves acquiring multiple holographic images of the sample at different positions of the acquisition focal plane and determining the biomass parameter value for each image. A distribution indicator is constructed from the biomass parameter values at the same measurement time and used to represent the distribution of biological agents in the sample.

  • The method involves acquiring holographic images of a biological sample at different positions of the acquisition focal plane.
  • Biomass parameter values are determined from each acquired holographic image, representing the quantity of biological agents at the corresponding position.
  • A distribution indicator is constructed from the biomass parameter values at the same measurement time.
  • The distribution indicator provides a representation of the distribution of the biomass of biological agents in the sample.
  • The analysis results include a representation of the biomass distribution derived from the distribution indicator.

Potential Applications:

  • Biomedical research: This method can be used to analyze biological samples in various research applications, such as studying cell growth, monitoring bacterial populations, or analyzing tissue samples.
  • Disease diagnosis: The method can aid in the diagnosis of diseases by analyzing biological samples for the presence and distribution of specific biomarkers or pathogens.
  • Environmental monitoring: It can be used to analyze samples from environmental sources, such as water or soil, to monitor the presence and distribution of microorganisms or pollutants.

Problems Solved:

  • Traditional methods of analyzing biological samples may require staining or labeling, which can alter the sample and introduce artifacts. This method allows for label-free analysis.
  • The method provides a non-invasive and non-destructive way of analyzing biological samples, preserving the integrity of the sample.
  • It allows for the analysis of the distribution of biological agents within the sample, providing valuable information about their spatial organization.

Benefits:

  • High-resolution analysis: Holographic imaging provides detailed information about the sample, allowing for the analysis of individual cells or microorganisms.
  • Label-free analysis: The method eliminates the need for staining or labeling, reducing sample preparation time and potential artifacts.
  • Non-invasive and non-destructive: The method does not harm the sample, making it suitable for longitudinal studies or analysis of precious samples.
  • Spatial distribution information: The method provides insights into the spatial organization of biological agents within the sample, which can be crucial for understanding their behavior and interactions.


Original Abstract Submitted

a method of analyzing a biological sample including biological agents and disposed in an analysis receptacle in a field of view of a holographic imaging system defining an acquisition focal plane, including, for each of a plurality of measurement times: acquiring a plurality of holographic images of the biological sample at different respective positions of the acquisition focal plane, and, from each acquired holographic image, determining a value of a biomass parameter representative of the quantity of biological agents at the position of the acquisition focal plane, the method including constructing a distribution indicator from values of the biomass parameter at the same measurement time for a plurality of positions of the acquisition focal plane, and providing, among the analysis results, a representation of the distribution of the biomass of biological agents, derived from at least one distribution indicator at a measurement time.

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