20240010999. ENGINEERED GLYCOSYLTRANSFERASES AND STEVIOL GLYCOSIDE GLUCOSYLATION METHODS simplified abstract (Codexis, Inc.)

ENGINEERED GLYCOSYLTRANSFERASES AND STEVIOL GLYCOSIDE GLUCOSYLATION METHODS

Organization Name

Codexis, Inc.

Inventor(s)

Jonathan Vroom of South San Francisco CA (US)

Stephanie Sue Galanie of Knoxville TN (US)

Jack Liang of South San Francisco CA (US)

Joyce Liu of Fremont CA (US)

Nikki Dellas of San Carlos CA (US)

Melissa Ann Mayo of Foster City CA (US)

David Entwistle of San Carlos CA (US)

ENGINEERED GLYCOSYLTRANSFERASES AND STEVIOL GLYCOSIDE GLUCOSYLATION METHODS - A simplified explanation of the abstract

This abstract first appeared for US patent application 20240010999 titled 'ENGINEERED GLYCOSYLTRANSFERASES AND STEVIOL GLYCOSIDE GLUCOSYLATION METHODS

Simplified Explanation

The present invention is related to engineered glycosyltransferase (gt) enzymes, polypeptides with gt activity, and polynucleotides encoding these enzymes. It also includes vectors and host cells containing these polynucleotides and polypeptides. Additionally, the invention provides engineered sucrose synthase (sus) enzymes, polypeptides with sus activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising them. Compositions comprising the gt enzymes and methods of using them to produce products with β-glucose linkages are also disclosed. The invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside m, rebaudioside a, rebaudioside i, and rebaudioside d). Methods for producing gt and sus enzymes are also included.

• Engineered glycosyltransferase (gt) enzymes, polypeptides, and polynucleotides are provided.
• Engineered sucrose synthase (sus) enzymes, polypeptides, and polynucleotides are also provided.
• Compositions comprising gt enzymes and methods for using them to produce products with β-glucose linkages are disclosed.
• Compositions and methods for the production of rebaudiosides are included.
• Methods for producing gt and sus enzymes are provided.

Potential Applications

This technology has potential applications in various industries, including:

• Food and beverage industry for the production of products with β-glucose linkages.
• Pharmaceutical industry for the production of rebaudiosides, which are natural sweeteners.
• Biotechnology industry for the development of engineered enzymes with specific activities.

Problems Solved

This technology addresses the following problems:

• Limited availability of enzymes with gt and sus activities for specific applications.
• Difficulty in producing rebaudiosides in large quantities using traditional methods.
• Lack of efficient methods for the production of products with β-glucose linkages.

Benefits

The benefits of this technology include:

• Increased availability of engineered enzymes with gt and sus activities.
• Enhanced production of rebaudiosides, which are natural sweeteners.
• Improved methods for the production of products with β-glucose linkages.

Original Abstract Submitted

the present invention provides engineered glycosyltransferase (gt) enzymes, polypeptides having gt activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. the present invention provides engineered sucrose synthase (sus) enzymes, polypeptides having sus activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. the present invention also provides compositions comprising the gt enzymes and methods of using the engineered gt enzymes to make products with �-glucose linkages. the present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside m, rebaudioside a, rebaudioside i, and rebaudioside d). the present invention also provides compositions comprising the sus enzymes and methods of using them. methods for producing gt and sus enzymes are also provided.