20240008461. CRUCIAN CARP (CARASSIUS AURATUS) STRAIN WITHOUT INTERMUSCULAR BONES AND BREEDING METHOD THEREOF simplified abstract (HEILONGJIANG RIVER FISHERIES RESEARCH INSTITUTE OF CHINESE ACADEMY OF FISHERY SCIENCES)

CRUCIAN CARP (CARASSIUS AURATUS) STRAIN WITHOUT INTERMUSCULAR BONES AND BREEDING METHOD THEREOF

Organization Name

HEILONGJIANG RIVER FISHERIES RESEARCH INSTITUTE OF CHINESE ACADEMY OF FISHERY SCIENCES

Inventor(s)

Youyi Kuang of Harbin, Heilongjiang (CN)

Xianhu Zheng of Harbin, Heilongjiang (CN)

Guangxiang Tong of Harbin, Heilongjiang (CN)

Zhipeng Sun of Harbin, Heilongjiang (CN)

Dingchen Cao of Harbin, Heilongjiang (CN)

Ting Yan of Harbin, Heilongjiang (CN)

Huan Xu of Harbin, Heilongjiang (CN)

Le Dong of Harbin, Heilongjiang (CN)

Xiaoxing Yang of Harbin, Heilongjiang (CN)

Tianqi Liu of Harbin, Heilongjiang (CN)

Tan Zhang of Harbin, Heilongjiang (CN)

Tingting Zhang of Harbin, Heilongjiang (CN)

Xiaowen Sun of Harbin, Heilongjiang (CN)

CRUCIAN CARP (CARASSIUS AURATUS) STRAIN WITHOUT INTERMUSCULAR BONES AND BREEDING METHOD THEREOF - A simplified explanation of the abstract

This abstract first appeared for US patent application 20240008461 titled 'CRUCIAN CARP (CARASSIUS AURATUS) STRAIN WITHOUT INTERMUSCULAR BONES AND BREEDING METHOD THEREOF

Simplified Explanation

The disclosed patent application describes a method for breeding strains of organisms without intermuscular bones. Here is a simplified explanation of the abstract:

• The method involves designing knockout target sites for two copies of the bmp6 gene (bmp6a and bmp6b) in the organism's genome.
• Through two rounds of gene knockout and screening, f2 generation individuals are obtained from a homozygous line with double gene mutation of bmp6a and bmp6b.
• The f2 generation individuals from the homozygous line are then used to propagate and form a new strain with intermuscular bone deficiency.
• The method allows for the creation of strains with less than 20 intermuscular bones or strains completely lacking intermuscular bones.

Potential Applications of this Technology:

• This technology could be applied in the field of biomedical research to study the role and function of intermuscular bones in organisms.
• It may also have applications in the development of animal models for studying bone-related diseases or conditions.

Problems Solved by this Technology:

• The method provides a way to selectively breed strains of organisms without intermuscular bones, allowing for the study of the effects of intermuscular bones on various biological processes.
• It eliminates the need for invasive procedures or surgical removal of intermuscular bones in organisms.

Benefits of this Technology:

• The method provides a controlled and efficient way to breed strains without intermuscular bones, saving time and resources compared to traditional breeding methods.
• It allows researchers to investigate the impact of intermuscular bones on organism development, movement, and other physiological processes.
• The technology may contribute to advancements in understanding bone biology and potential therapeutic interventions for bone-related disorders.

Original Abstract Submitted

the disclosure provides a method for breeding strains without intermuscular bones, which comprises designing knockout target sites for the two copies of the bmp6, bmp6a and bmp6b, in the genome, and then obtaining f2 generation individuals from a homozygous line with bmp6a and bmp6b double gene mutation through two rounds of gene knockout and screening, and then propagating by using the f2 generation individuals from the homozygous line with bmp6a and bmp6b double gene mutation to form a new strain with intermuscular bone-deficient. strains of with less than 20 intermuscular bones and without intermuscular bones are obtained by the method of the present disclosure.