18459484. METHODS AND COMPOSITIONS FOR GENERATING COMPLEX TRAIT LOCI simplified abstract (PIONEER HI-BRED INTERNATIONAL, INC.)

METHODS AND COMPOSITIONS FOR GENERATING COMPLEX TRAIT LOCI

Organization Name

PIONEER HI-BRED INTERNATIONAL, INC.

Inventor(s)

SAVERIO CARL Falco of WILMINGTON DE (US)

MICHAEL Lassnser of PORTLAND OR (US)

ZHONGSEN Li of HOCKESSIN DE (US)

CHRISTOPHER J. Scelonge of ANKENY IA (US)

METHODS AND COMPOSITIONS FOR GENERATING COMPLEX TRAIT LOCI - A simplified explanation of the abstract

This abstract first appeared for US patent application 18459484 titled 'METHODS AND COMPOSITIONS FOR GENERATING COMPLEX TRAIT LOCI

Simplified Explanation

Compositions and methods are provided for stacking multiple independent transgenic loci into the genome of a plant. Compositions include plants, seeds or plant cells comprising at least one transgenic target site and at least one genomic locus of interest integrated at different genomic sites within a genomic window. Plant breeding techniques can be employed such that the transgenic target site and the genomic locus of interest can be bred together. In this way, multiple independent transgene integrations can be generated within a genomic window to create a complex trait locus. The complex trait locus is designed such that the transgenic target sites and/or genomic loci of interest can segregate independently of each other, thus providing the benefit of altering a complex trait locus by breeding-in and breeding-away specific elements. Various methods can also be employed to modify the target sites such that they contain a variety of polynucleotides of interest.

• Plants, seeds, or plant cells can be modified to contain multiple independent transgenic loci within a genomic window.
• Plant breeding techniques can be used to breed together transgenic target sites and genomic loci of interest.
• The complex trait locus created allows for independent segregation of transgenic target sites and genomic loci of interest, enabling specific elements to be bred-in or bred-away.

Potential Applications

This technology could be applied in agriculture for creating plants with complex traits, such as disease resistance or improved nutritional content.

Problems Solved

This technology solves the problem of efficiently stacking multiple transgenic loci in plants to create complex trait loci that can be independently segregated.

Benefits

The benefits of this technology include the ability to modify complex trait loci in plants by breeding-in or breeding-away specific elements, leading to improved plant characteristics.

Potential Commercial Applications

Potential commercial applications of this technology include the development of genetically modified crops with enhanced traits for agricultural purposes.

Possible Prior Art

One possible prior art could be the use of traditional breeding techniques to introduce single transgenic loci into plants, but not specifically stacking multiple independent transgenic loci within a genomic window.

How does this technology compare to other methods of stacking transgenic loci in plants?

This article does not provide a comparison with other methods of stacking transgenic loci in plants, such as CRISPR/Cas9-mediated genome editing or traditional breeding techniques.

What are the potential regulatory challenges associated with the commercialization of plants generated using this technology?

This article does not address the potential regulatory challenges that may arise when commercializing plants generated using this technology, such as GMO regulations and public acceptance.

Original Abstract Submitted

Compositions and methods are provided for stacking multiple independent transgenic loci into the genome of a plant. Compositions include plants, seeds or plant cells comprising at least one transgenic target site and at least one genomic locus of interest integrated at different genomic sites within a genomic window. Plant breeding techniques can be employed such that the transgenic target site and the genomic locus of interest can be bred together. In this way, multiple independent transgene integrations can be generated within a genomic window to create a complex trait locus. The complex trait locus is designed such that the transgenic target sites and/or genomic loci of interest can segregate independently of each other, thus providing the benefit of altering a complex trait locus by breeding-in and breeding-away specific elements. Various methods can also be employed to modify the target sites such that they contain a variety of polynucleotides of interest.