18306653. DE NOVO POLYNUCLEOTIDE SYNTHESIS WITH SUBSTRATE-BOUND POLYMERASE simplified abstract (MICROSOFT TECHNOLOGY LICENSING, LLC)
Contents
- 1 DE NOVO POLYNUCLEOTIDE SYNTHESIS WITH SUBSTRATE-BOUND POLYMERASE
- 1.1 Organization Name
- 1.2 Inventor(s)
- 1.3 DE NOVO POLYNUCLEOTIDE SYNTHESIS WITH SUBSTRATE-BOUND POLYMERASE - A simplified explanation of the abstract
- 1.4 Simplified Explanation
- 1.5 Potential Applications
- 1.6 Problems Solved
- 1.7 Benefits
- 1.8 Potential Commercial Applications
- 1.9 Possible Prior Art
- 1.10 Original Abstract Submitted
DE NOVO POLYNUCLEOTIDE SYNTHESIS WITH SUBSTRATE-BOUND POLYMERASE
Organization Name
MICROSOFT TECHNOLOGY LICENSING, LLC
Inventor(s)
Bichlien Nguyen of Seattle WA (US)
Karin Strauss of Seattle WA (US)
DE NOVO POLYNUCLEOTIDE SYNTHESIS WITH SUBSTRATE-BOUND POLYMERASE - A simplified explanation of the abstract
This abstract first appeared for US patent application 18306653 titled 'DE NOVO POLYNUCLEOTIDE SYNTHESIS WITH SUBSTRATE-BOUND POLYMERASE
Simplified Explanation
The abstract describes a patent application for de novo polynucleotide synthesis using a substrate-bound polymerase attached to a solid substrate, such as a microelectrode array. The polymerase adds nucleotides to growing polynucleotide strands that are also attached to the solid substrate, with spatial control achieved by changing the rate of nucleotide polymerization at selected locations on the surface of the substrate.
- The polymerase is attached to a solid substrate, allowing for spatial control of polymerase activity.
- Nucleotides are added to growing polynucleotide strands at specific locations on the substrate.
- The rate of polymerization can be changed by inhibiting or promoting polymerase activity.
- Activation of electrodes in the microelectrode array can alter the rate of nucleotide polymerization.
- By varying the locations of active polymerase and the nucleotide species added, polynucleotides with different sequences can be synthesized on the solid substrate.
Potential Applications
This technology could be applied in:
- DNA sequencing
- Drug development
- Genetic engineering
Problems Solved
- Controlled synthesis of polynucleotides
- Spatially controlled polymerase activity
- Generation of polynucleotides with arbitrary sequences
Benefits
- Precise control over polynucleotide synthesis
- Ability to create custom sequences
- Potential for high-throughput applications
Potential Commercial Applications
- DNA Sequencing Technology: Advancements and Innovations
Possible Prior Art
No known prior art at this time.
Unanswered Questions
How does this technology compare to traditional methods of polynucleotide synthesis?
This technology offers spatial control over polymerase activity, allowing for precise manipulation of nucleotide addition. Traditional methods may not provide the same level of control.
What are the limitations of this technology in terms of sequence length and complexity?
The abstract does not specify the maximum sequence length or complexity that can be achieved using this technology. Further research may be needed to determine these limitations.
Original Abstract Submitted
De novo polynucleotide synthesis is performed with a substrate-bound polymerase. The polymerase is attached to a solid substrate such as a microelectrode array. The polymerase adds nucleotides to growing polynucleotides strands that are also attached to the solid substrate. Spatial control of polymerase activity is achieved by changing the rate of nucleotide polymerization at selected locations on the surface of the solid substrate. The rate of polymerization is changed by inhibiting or promoting activity of the polymerase. In some implementations, activation of electrodes in the microelectrode array changes the rate of nucleotide polymerization. Nucleotides are added to the growing polynucleotide strands at areas where the polymerase is active. By varying the locations where the substrate-bound polymerase is active and the species of nucleotide added, a population of polynucleotides with different, arbitrary sequences is synthesized on the surface of the solid substrate.
