18157449. LINKER STRUCTURES WITH MINIMAL SCAR FOR ENZYMATIC SYNTHESIS simplified abstract (MICROSOFT TECHNOLOGY LICENSING, LLC)
Contents
LINKER STRUCTURES WITH MINIMAL SCAR FOR ENZYMATIC SYNTHESIS
Organization Name
MICROSOFT TECHNOLOGY LICENSING, LLC
Inventor(s)
Bichlien Hoang Nguyen of Seattle WA (US)
LINKER STRUCTURES WITH MINIMAL SCAR FOR ENZYMATIC SYNTHESIS - A simplified explanation of the abstract
This abstract first appeared for US patent application 18157449 titled 'LINKER STRUCTURES WITH MINIMAL SCAR FOR ENZYMATIC SYNTHESIS
Simplified Explanation
The patent application describes electrochemically-cleavable linkers that can be used to attach a nucleotide to a bound group. These linkers have cleavage potentials that are lower than the redox potential of the solvent, which can be water or an aqueous buffer solution.
- Electrochemically-cleavable linkers with cleavage potentials lower than the redox potential of the solvent
- Linkers can be used to attach a nucleotide to a bound group
- Cleavable groups include methoxybenzyl alcohol, ester, propargyl thioether, or trichloroethyl ether
- Linkers can be cleaved in solvent by generating an electrode potential lower than the redox potential of the solvent
- Electrode array can be used to generate localized electrode potentials for selective cleavage of linkers
Potential applications of this technology:
- Attachment of blocking groups to nucleotides in enzymatic oligonucleotide synthesis
- Controlled release of nucleotides in drug delivery systems
- Selective cleavage of linkers in bioconjugation reactions
Problems solved by this technology:
- Previous linkers had cleavage potentials higher than the redox potential of the solvent, limiting their use in aqueous environments
- Electrode array allows for localized cleavage of linkers, minimizing damage to surrounding molecules
Benefits of this technology:
- Enables efficient and selective cleavage of linkers in aqueous environments
- Provides a versatile tool for attaching nucleotides to bound groups in various applications
- Allows for controlled release of nucleotides in drug delivery systems, improving therapeutic efficacy
Original Abstract Submitted
This disclosure provides electrochemically-cleavable linkers with cleavage potentials that are less than the redox potential of the solvent in which the linkers are used. In some applications, the solvent may be water or an aqueous buffer solution. The linkers may be used to link a nucleotide to a bound group. The linkers include a cleavable group which may be one of a methoxybenzyl alcohol, an ester, a propargyl thioether, or a trichloroethyl ether. The linkers may be cleaved in solvent by generating an electrode potential that is less than the redox potential of the solvent. In some implementations, an electrode array may be used to generate localized electrode potentials which selectively cleave linkers bound to the activated electrode. Uses for the linkers include attachment of blocking groups to nucleotides in enzymatic oligonucleotide synthesis.
