18130202. RNA-GUIDED ENDONUCLEASE FUSION POLYPEPTIDES AND METHODS OF USE THEREOF simplified abstract (THE REGENTS OF THE UNIVERSITY OF CALIFORNIA)
RNA-GUIDED ENDONUCLEASE FUSION POLYPEPTIDES AND METHODS OF USE THEREOF
Organization Name
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor(s)
Schaked Omer Halperin of Berkeley CA (US)
RNA-GUIDED ENDONUCLEASE FUSION POLYPEPTIDES AND METHODS OF USE THEREOF - A simplified explanation of the abstract
This abstract first appeared for US patent application 18130202 titled 'RNA-GUIDED ENDONUCLEASE FUSION POLYPEPTIDES AND METHODS OF USE THEREOF
Simplified Explanation
The present patent application describes a fusion polypeptide that combines an RNA-guided endonuclease and an error-prone DNA polymerase. This fusion polypeptide is used in a system with a guide RNA to introduce mutations in a target DNA sequence. The application also includes a method for mutagenizing a target polynucleotide using this fusion polypeptide.
- Fusion polypeptide: The patent application describes a fusion polypeptide that combines an RNA-guided endonuclease and an error-prone DNA polymerase.
- System: The fusion polypeptide is used in a system with a guide RNA to introduce mutations in a target DNA sequence.
- Cell: The patent application also includes a cell that contains the fusion polypeptide or the system described above.
- Method: The application provides a method for mutagenizing a target polynucleotide using the fusion polypeptide.
Potential Applications
This technology has potential applications in various fields, including:
- Genetic research: The fusion polypeptide and system described in the patent application can be used to introduce specific mutations in target DNA sequences, allowing researchers to study the effects of these mutations on gene function.
- Biotechnology: The ability to introduce targeted mutations in DNA sequences can be valuable in biotechnology applications, such as engineering enzymes or modifying organisms for specific purposes.
- Drug development: This technology can be used to create specific mutations in target genes to study their role in disease development or to develop targeted therapies.
Problems Solved
This technology addresses several problems in genetic research and biotechnology:
- Targeted mutagenesis: The fusion polypeptide and system described in the patent application provide a precise and efficient method for introducing mutations in target DNA sequences.
- Error-prone DNA polymerase: The inclusion of an error-prone DNA polymerase in the fusion polypeptide allows for the introduction of random mutations, expanding the range of mutations that can be generated.
- Simplified process: The fusion polypeptide and system described in the patent application streamline the process of mutagenizing target polynucleotides, making it more accessible and efficient for researchers.
Benefits
The use of this technology offers several benefits:
- Precision: The fusion polypeptide and system allow for precise targeting of specific DNA sequences, ensuring that mutations are introduced at the desired locations.
- Efficiency: The fusion polypeptide and system provide an efficient method for introducing mutations, reducing the time and resources required for genetic research and biotechnology applications.
- Versatility: The inclusion of an error-prone DNA polymerase in the fusion polypeptide allows for the generation of a wide range of mutations, increasing the versatility of this technology for various applications.
Original Abstract Submitted
The present disclosure provides a fusion polypeptide comprising: a) an enzymatically active RNA-guided endonuclease that introduces a single-stranded break in a target DNA; and b) an error-prone DNA polymerase. The present disclosure provides a system comprising: a) a fusion polypeptide of the present disclosure; and b) a guide RNA. The present disclosure provides a cell comprising a fusion polypeptide of the present disclosure, or a system of the present disclosure. The present disclosure provides a method of mutagenizing a target polynucleotide.
