18130199. RNA-GUIDED ENDONUCLEASE FUSION POLYPEPTIDES AND METHODS OF USE THEREOF simplified abstract (THE REGENTS OF THE UNIVERSITY OF CALIFORNIA)
Contents
RNA-GUIDED ENDONUCLEASE FUSION POLYPEPTIDES AND METHODS OF USE THEREOF
Organization Name
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor(s)
Schaked Omer Halperin of Berkeley CA (US)
RNA-GUIDED ENDONUCLEASE FUSION POLYPEPTIDES AND METHODS OF USE THEREOF - A simplified explanation of the abstract
This abstract first appeared for US patent application 18130199 titled 'RNA-GUIDED ENDONUCLEASE FUSION POLYPEPTIDES AND METHODS OF USE THEREOF
Simplified Explanation
The present patent application describes a fusion polypeptide that combines an RNA-guided endonuclease and an error-prone DNA polymerase. This fusion polypeptide is used in a system along with a guide RNA to introduce mutations in a target DNA sequence. The application also describes a method of mutagenizing a target polynucleotide using this fusion polypeptide system.
- Fusion polypeptide: A combination of an RNA-guided endonuclease and an error-prone DNA polymerase.
- System: The fusion polypeptide is used together with a guide RNA to introduce mutations in a target DNA sequence.
- Cell: The patent application also covers a cell that contains the fusion polypeptide or the fusion polypeptide system.
- Method: The application describes a method of mutagenizing a target polynucleotide using the fusion polypeptide system.
Potential applications of this technology:
- Genetic engineering: The fusion polypeptide system can be used to introduce specific mutations in target DNA sequences, allowing for precise genetic modifications.
- Biomedical research: This technology can be used to study the effects of specific mutations on gene function and disease development.
- Drug discovery: The ability to introduce mutations in target DNA sequences can aid in the development of new drugs by studying the effects of these mutations on drug efficacy and resistance.
Problems solved by this technology:
- Precise mutagenesis: The fusion polypeptide system allows for targeted introduction of mutations in DNA sequences, providing a more precise and controlled method compared to traditional mutagenesis techniques.
- Efficiency: The fusion polypeptide system combines the activities of an RNA-guided endonuclease and an error-prone DNA polymerase, increasing the efficiency of mutagenesis.
- Specificity: The guide RNA directs the fusion polypeptide to the desired target DNA sequence, ensuring that mutations are introduced at the intended location.
Benefits of this technology:
- Precision: The fusion polypeptide system allows for precise introduction of mutations at specific locations in DNA sequences.
- Efficiency: The combination of an RNA-guided endonuclease and an error-prone DNA polymerase increases the efficiency of mutagenesis.
- Versatility: The fusion polypeptide system can be used in various applications, including genetic engineering, biomedical research, and drug discovery.
Original Abstract Submitted
The present disclosure provides a fusion polypeptide comprising: a) an enzymatically active RNA-guided endonuclease that introduces a single-stranded break in a target DNA; and b) an error-prone DNA polymerase. The present disclosure provides a system comprising: a) a fusion polypeptide of the present disclosure; and b) a guide RNA. The present disclosure provides a cell comprising a fusion polypeptide of the present disclosure, or a system of the present disclosure. The present disclosure provides a method of mutagenizing a target polynucleotide.
