17767844. METHODS TO STABILIZE MAMMALIAN CELLS simplified abstract (THE REGENTS OF THE UNIVERSITY OF CALIFORNIA)

METHODS TO STABILIZE MAMMALIAN CELLS

Organization Name

THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor(s)

Nathan E. Lewis of San Diego CA (US)

Philipp Spahn of San Diego CA (US)

Shangzhong Li of Cambridge MA (US)

Hooman Hefzi of Berkeley CA (US)

Isaac Shamie of La Jolla CA (US)

METHODS TO STABILIZE MAMMALIAN CELLS - A simplified explanation of the abstract

This abstract first appeared for US patent application 17767844 titled 'METHODS TO STABILIZE MAMMALIAN CELLS

Simplified Explanation

The invention provides gene targets whose restoration leads to genome stabilization in host cells, such as Chinese Hamster Ovary (CHO) cells. Many DNA repair genes are mutated in CHO cells which compromises their ability to repair naturally occurring DNA damage, in particular double-strand breaks (DSBs). Unrepaired DSBs can give rise to chromosomal instability which, in turn, can lead to loss of transgenes from the genome. As a consequence, protein titer can drop significantly, rendering protein production unprofitable. The invention provides a set of mutated DNA repair genes whose restoration yields significant improvement in DSB repair, genome stability, and protein titer.

• Gene targets identified for genome stabilization in host cells like CHO cells
• Mutated DNA repair genes in CHO cells compromise their ability to repair DSBs
• Restoration of mutated DNA repair genes leads to improved DSB repair and genome stability
• Loss of transgenes due to chromosomal instability can impact protein titer

Potential Applications

The technology can be applied in biopharmaceutical production, gene therapy, and genetic engineering.

Problems Solved

The technology addresses the issue of genome instability and reduced protein production efficiency in host cells like CHO cells.

Benefits

The restoration of gene targets leads to improved DSB repair, genome stability, and protein titer, resulting in more efficient protein production processes.

Potential Commercial Applications

The technology can be utilized in the biopharmaceutical industry for the production of therapeutic proteins and antibodies.

Possible Prior Art

Prior research has focused on DNA repair mechanisms in various cell types, but specific gene targets for genome stabilization in CHO cells may be novel.

How does the restoration of mutated DNA repair genes impact overall cell health and viability in CHO cells?

The article does not delve into the potential effects of restoring mutated DNA repair genes on the overall health and viability of CHO cells. Further research may be needed to understand the broader implications of this genetic intervention.

What are the specific mechanisms by which the identified gene targets improve DSB repair and genome stability in host cells?

The article provides an overview of the benefits of restoring mutated DNA repair genes but does not detail the specific molecular mechanisms involved in enhancing DSB repair and genome stability. Further studies could elucidate the precise pathways through which these gene targets exert their effects.

Original Abstract Submitted

The invention provides gene targets whose restoration leads to genome stabilization in host cells, such as Chinese Hamster Ovary (CHO) cells. Many DNA repair genes are mutated in CHO cells which compromises their ability to repair naturally occurring DNA damage, in particular double-strand breaks (DSBs). Unrepaired DSBs can give rise to chromosomal instability which, in turn, can lead to loss of transgenes from the genome. As a consequence, protein titer can drop significantly, rendering protein production unprofitable. The invention provides a set of mutated DNA repair genes whose restoration yields significant improvement in DSB repair, genome stability, and protein titer.