17754890. DETECTION OF SEQUENCES UNIQUELY ASSOCIATED WITH A DNA TARGET REGION simplified abstract (PIONEER HI-BRED INTERNATIONAL, INC.)

DETECTION OF SEQUENCES UNIQUELY ASSOCIATED WITH A DNA TARGET REGION

Organization Name

PIONEER HI-BRED INTERNATIONAL, INC.

Inventor(s)

ANANTA Acharya of JOHNSTON IA (US)

AMANDA MARIE Gannon of DALLAS CENTER IA (US)

HENA Guo of JOHNSTON IA (US)

KEVIN Hayes of URBANDALE IA (US)

ROBYN LYNN Laskowski of DES MOINES IA (US)

ROBERT EBOW Mcewan of ANKENY IA (US)

CARISA Townsend of PALMETTO GA (US)

LASANTHA Ubayasena of WEST LAFAYETTE IN (US)

GINA MARIE Zastrow-hayes of URBANDALE IA (US)

DETECTION OF SEQUENCES UNIQUELY ASSOCIATED WITH A DNA TARGET REGION - A simplified explanation of the abstract

This abstract first appeared for US patent application 17754890 titled 'DETECTION OF SEQUENCES UNIQUELY ASSOCIATED WITH A DNA TARGET REGION

Simplified Explanation

The disclosed embodiments involve methods for determining sequences of interest in plants using targeted unique molecular index (TUMI) sequences. These TUMI sequences are uniquely associated with individual polynucleotide fragments, such as those found in transgenic events, site-specific mutations, or wild type variants. System, apparatus, and computer program products are also provided for implementing these methods.

• TUMI sequences uniquely associated with individual polynucleotide fragments in plants
• Methods for determining sequences of interest in plants
• System, apparatus, and computer program products provided for implementing the methods

Potential Applications

The technology can be applied in:

• Plant breeding programs
• Genetic engineering research
• Crop improvement studies

Problems Solved

This technology helps in:

• Identifying specific sequences in plants efficiently
• Differentiating between different polynucleotide fragments accurately

Benefits

The benefits of this technology include:

• Enhanced precision in sequence determination
• Increased efficiency in analyzing plant genetic material
• Facilitates targeted genetic modifications

Potential Commercial Applications

This technology could be valuable in:

• Agriculture biotechnology companies
• Seed production industries
• Research institutions focusing on plant genetics

Possible Prior Art

One possible prior art could be the use of unique molecular identifiers (UMIs) in sequencing technologies to improve accuracy and reduce errors in identifying DNA sequences.

What are the limitations of this technology in terms of scalability and cost-effectiveness?

This technology may face challenges in scaling up for large-scale plant sequencing projects due to the cost associated with implementing TUMI sequences for each polynucleotide fragment.

How does this technology compare to existing methods for determining sequences of interest in plants?

Compared to traditional sequencing methods, the use of TUMI sequences offers higher specificity and accuracy in identifying individual polynucleotide fragments in plants.

Original Abstract Submitted

The disclosed embodiments concern methods for determining sequences of interest using targeted unique molecular index (TUMI) sequences that are uniquely associated with individual polynucleotide fragments in plants, such as those present in a transgenic event, a site-specific mutation or a wild type variant. System, apparatus, and computer program products are also provided for determining a sequence of interest implementing the methods disclosed.