18560106. METHODS OF ANALYZING SHAPED PARTICLES CONTAINING CELLS USING FLUORESCENCE ACTIVATED CELL SORTING simplified abstract (The Regents of the University of California)

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METHODS OF ANALYZING SHAPED PARTICLES CONTAINING CELLS USING FLUORESCENCE ACTIVATED CELL SORTING

Organization Name

The Regents of the University of California

Inventor(s)

Dino Di Carlo of Los Angeles CA (US)

Joseph De Rutte of Los Angeles CA (US)

Robert Dimatteo of Sherman Oaks CA (US)

METHODS OF ANALYZING SHAPED PARTICLES CONTAINING CELLS USING FLUORESCENCE ACTIVATED CELL SORTING - A simplified explanation of the abstract

This abstract first appeared for US patent application 18560106 titled 'METHODS OF ANALYZING SHAPED PARTICLES CONTAINING CELLS USING FLUORESCENCE ACTIVATED CELL SORTING

The method described in the patent application involves analyzing shaped particles using a flow cytometer or a fluorescence activated cell sorter (FACS) by flowing a population of shaped particles through the instrument and optically interrogating them to measure scattered light and fluorescence signals.

  • Flowing a population of shaped particles through a flow cytometer or FACS.
  • Optically interrogating the shaped particles to measure scattered light and fluorescence signals.
  • Detecting target shaped particles based on measurements of scattered light or fluorescence signal levels.
  • Optimizing sorting of target shaped particles by adjusting drop delay or sorting mask configuration.

Potential Applications: - Biomedical research for analyzing cells and particles. - Pharmaceutical industry for drug development and quality control. - Environmental monitoring for analyzing pollutants or microorganisms.

Problems Solved: - Efficient and accurate analysis of shaped particles. - Identification and sorting of target particles. - Optimization of flow cytometer or FACS settings for better results.

Benefits: - Improved accuracy and efficiency in particle analysis. - Enhanced capabilities for cell sorting and identification. - Potential for increased productivity in research and industrial applications.

Commercial Applications: Title: "Advanced Particle Analysis Technology for Biomedical and Pharmaceutical Industries" This technology can be used in research laboratories, pharmaceutical companies, and environmental monitoring agencies for various applications such as cell analysis, drug development, and pollution monitoring.

Prior Art: Researchers can explore existing patents related to flow cytometry, cell sorting, and particle analysis technologies to understand the background of this innovation.

Frequently Updated Research: Scientists are continuously working on improving flow cytometry and FACS technologies for better accuracy and efficiency in particle analysis.

Questions about Shaped Particle Analysis: 1. How does this technology improve the efficiency of particle analysis compared to traditional methods? - This technology allows for high-throughput analysis and accurate identification of target particles, leading to faster and more reliable results. 2. What are the potential challenges in implementing this technology in different industries? - Some challenges may include the need for specialized training to operate flow cytometers or FACS systems and the initial investment required for acquiring the technology.


Original Abstract Submitted

A method of analyzing shaped particles using a flow cytometer or a fluorescence activated cell sorter (FACS) includes flowing a population of shaped particles with at least some of the population of shaped particles having cells loaded therein through the flow cytometer or FACS and optically interrogating the shaped particles in the flow cytometer or FACS to measure scattered light for each shaped particle. A target shaped particle having a cell loaded therein is detected based at least in part on a measurement of forward scattered light, side scattered light, or back scattered light. The target shaped particle may also be identified with a measured fluorescence signal level. Sorting of target shaped particles may be optimized by adjusting one or more of a drop delay or a sorting mask configuration for the flow cytometer or FACS.