ENHANCED RECOVERY AND ISOLATION OF TARGET CELLS FROM BLOOD SAMPLES

Abstract: the invention relates to a method and platform for producing car-t cells and processing biological cells within a single, functionally closed container. the method involves introducing host cells into a sealed container with multiple aseptic passages, performing genetic modification, and maintaining cells in controlled conditions monitored by real-time feedback until a desired fraction of genetically modified cells is achieved. the container also supports target cell isolation using buoyant microbubbles functionalized with antibodies or aptamers, facilitating separation of target cells from non-target cells during centrifugation. pneumatic pressure is employed for waste separation and cell harvest, achieving high-purity cell recovery. the platform includes modules for cell enrichment, sequestration, and isolation, achieving high recovery and purity rates for therapeutic cell populations. this integrated, closed-system approach ensures aseptic conditions, efficient processing, and high-quality cell products for advanced therapeutic applications.

Inventor(s): Jonathan Ellis, Philip H. Coelho

CPC Classification: C12N5/0081 (Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor; (plant reproduction by tissue culture techniques ))

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