QUANTITATIVE POLYMERASE CHAIN REACTION (QPCR) MICROFLUIDIC CHIP CARD AND REAL-TIME FLUORESCENCE POLYMERASE CHAIN REACTION (PCR) ANALYZER

Abstract: the present disclosure relates to a quantitative polymerase chain reaction (qpcr) microfluidic chip card and a real-time fluorescence pcr analyzer. the qpcr microfluidic chip card includes: a microfluidic chip body; a sample pretreatment portion; a reaction chamber, where the reaction chamber is in communication with a mixing portion; a first exhaust flow channel, configured for communicating the reaction chamber to the outside for gas discharge from the reaction chamber; and a first air-permeable water barrier member, located between the first exhaust flow channel and the reaction chamber. the microfluidic chip body includes a blocking region, at least a part of the first exhaust flow channel is located in the blocking region, and the part of the first exhaust flow channel in the blocking region is sealed by heating and/or extrusion deformation.

Inventor(s): Gangyi CHEN, Biao MU, Luwei YE, Xianghong ZENG, Wengui FU

CPC Classification: B01L3/502761 ({specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules (investigating characteristics of particles )})

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