20240052357. AUTONOMOUS INDUCIBLE DIRECTED EVOLUTION OF COMPLEX PATHWAYS simplified abstract (North Carolina State University)

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AUTONOMOUS INDUCIBLE DIRECTED EVOLUTION OF COMPLEX PATHWAYS

Organization Name

North Carolina State University

Inventor(s)

Nathan Crook of Raleigh NC (US)

Ibrahim Al'abri of Raleigh NC (US)

AUTONOMOUS INDUCIBLE DIRECTED EVOLUTION OF COMPLEX PATHWAYS - A simplified explanation of the abstract

This abstract first appeared for US patent application 20240052357 titled 'AUTONOMOUS INDUCIBLE DIRECTED EVOLUTION OF COMPLEX PATHWAYS

Simplified Explanation

The abstract describes a method for directed evolution in a microbe using a propagation deficient phage genome and a vector containing a target gene sequence. The method involves introducing these components into a first host cell, exposing the cell to a mutagenesis agent, inducing lysis of the cell to produce a lysate containing phage particles with the mutated target gene sequence, and infecting a second host cell with the lysate.

  • The method involves introducing a propagation deficient phage genome and a vector with a target gene sequence into a host cell.
  • The host cell is exposed to a mutagenesis agent to induce mutations in the target gene sequence.
  • Lysis of the host cell is induced, resulting in a lysate containing phage particles with the mutated target gene sequence.
  • The lysate is used to infect a second host cell.

Potential applications of this technology:

  • Directed evolution of genes: This method can be used to evolve specific genes in a controlled manner, allowing for the generation of novel variants with desired properties.
  • Protein engineering: By mutating the target gene sequence, this method can be used to engineer proteins with improved functionality or new properties.
  • Drug discovery: The directed evolution of genes can be applied to identify new drug targets or optimize existing drug targets for improved efficacy.

Problems solved by this technology:

  • Limited natural variation: This method allows for the generation of a diverse library of gene variants, overcoming the limitation of natural genetic variation.
  • Time and cost efficiency: Directed evolution can be a time-consuming and expensive process. This method provides a more efficient and streamlined approach to achieve desired genetic changes.

Benefits of this technology:

  • Controlled evolution: The method allows for the directed evolution of genes, enabling researchers to specifically target and modify desired gene sequences.
  • High throughput screening: The use of phage particles in the lysate allows for efficient screening of a large number of gene variants in a short period, facilitating the identification of desired traits or properties.
  • Versatility: The method can be applied to various microorganisms and target genes, making it a versatile tool for genetic engineering and evolution studies.


Original Abstract Submitted

a method for directed evolution in a microbe, the method comprising: introducing into a first host cell a propagation deficient phage genome and a vector comprising a target gene sequence to be mutated and a phage propagation component responsive to induce lysis of the first host cell and to provide for propagation of the phage genome; exposing the first host cell to a mutagenesis agent; inducing lysis of the first host cell and phage propagation to produce a lysate comprising phage particles comprising the target gene sequence; and infecting a second host cell with the lysate. systems and kits for practicing the method are also disclosed.

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