20240027325. METHOD AND APPARATUS FOR FLOW-BASED, SINGLE-PARTICLE AND/OR SINGLE-MOLECULE ANALYSIS simplified abstract (University of Washington)

METHOD AND APPARATUS FOR FLOW-BASED, SINGLE-PARTICLE AND/OR SINGLE-MOLECULE ANALYSIS

Organization Name

University of Washington

Inventor(s)

Daniel T. Chiu of Seattle WA (US)

Yifei Jiang of Seattle WA (US)

Mengxia Zhao of Seattle WA (US)

Yuanhua Cheng of Seattle WA (US)

METHOD AND APPARATUS FOR FLOW-BASED, SINGLE-PARTICLE AND/OR SINGLE-MOLECULE ANALYSIS - A simplified explanation of the abstract

This abstract first appeared for US patent application 20240027325 titled 'METHOD AND APPARATUS FOR FLOW-BASED, SINGLE-PARTICLE AND/OR SINGLE-MOLECULE ANALYSIS

Simplified Explanation

The patent application describes systems and methods for analyzing particles flowing in a channel. The channel is designed to flow a particle through a lumen, with an interrogation window allowing light to pass into and out of the lumen. The system includes a light engine with a first light source positioned to emit first excitation light onto a first portion of the channel in the interrogation window, and a second light source positioned to emit second excitation light onto a second portion of the channel in the interrogation window.

• The system includes an emission fiber bundle with a first and second emission optical fiber arranged in a bundle head.
• The first light source emits first excitation light onto a first portion of the channel, while the second light source emits second excitation light onto a separate second portion of the channel.
• The emission fiber bundle collects emitted light from the particles in the channel for analysis.

Potential applications of this technology:

• Particle analysis in medical diagnostics, such as blood cell counting or detecting pathogens.
• Environmental monitoring, such as analyzing pollutants or microorganisms in water samples.
• Industrial applications, such as analyzing particles in manufacturing processes or quality control.

Problems solved by this technology:

• Enables real-time analysis of particles flowing in a channel, providing valuable information for various applications.
• Allows for simultaneous analysis of different portions of the channel, increasing efficiency and accuracy.
• Provides a compact and integrated system for particle analysis.

Benefits of this technology:

• Faster and more efficient particle analysis compared to traditional methods.
• Real-time monitoring and analysis of particles in a flowing system.
• Compact and integrated design for easy implementation in various settings.
• Simultaneous analysis of multiple portions of the channel for improved accuracy.

Original Abstract Submitted

systems and methods for analyzing particles flowing in a channel are described. in an embodiment, the channel is configured to flow a particle through a lumen of the channel, the channel defining an interrogation window configured to allow light to pass into and out of the lumen; a light engine comprising: a first light source positioned to output first excitation light onto a first portion of the channel in the interrogation window; and a second light source positioned to output second excitation light onto a second portion of the channel in the interrogation window separate from the first portion. in an embodiment, the systems include an emission fiber bundle comprising a first emission optical fiber and a second emission optical fiber, wherein a proximal end of first emission optical fiber and second emission optical fiber are arranged in an emission fiber bundle head.