18377236. CHEMICALLY REVERSIBLE 2`-OH ACYLATION PROTECTS RNA FROM HYDROLYTIC AND ENZYMATIC DEGRADATION simplified abstract (THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY)
Contents
CHEMICALLY REVERSIBLE 2`-OH ACYLATION PROTECTS RNA FROM HYDROLYTIC AND ENZYMATIC DEGRADATION
Organization Name
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
Inventor(s)
Linglan Fang of Stanford CA (US)
Eric T. Kool of Stanford CA (US)
CHEMICALLY REVERSIBLE 2`-OH ACYLATION PROTECTS RNA FROM HYDROLYTIC AND ENZYMATIC DEGRADATION - A simplified explanation of the abstract
This abstract first appeared for US patent application 18377236 titled 'CHEMICALLY REVERSIBLE 2`-OH ACYLATION PROTECTS RNA FROM HYDROLYTIC AND ENZYMATIC DEGRADATION
Simplified Explanation
The patent application describes compositions and methods for modifying RNA to enhance its stability and functionality. Here are some key points from the abstract:
- RNA can be modified using acylimidazoles, sulfonyltriazoles, or sulfonylimidazoles to protect it from degradation.
- 2′-OH acylation helps RNA resist hydrolytic and enzymatic degradation.
- Water-soluble organocatalysts can accelerate the reversal of acylation adducts to restore RNA functionality.
- Chemically tuned 2′-OH acylation can be spontaneously released in cells to restore RNA biological functions, including translation.
- mRNA can be selectively modified at the 2′-OH of poly(A)-tail for enhanced stability and increased protein output.
---
- Potential Applications
This technology could be applied in the development of RNA-based therapeutics, gene editing tools, and RNA vaccines.
- Problems Solved
This technology addresses the issue of RNA degradation, which can limit the effectiveness of RNA-based therapies and vaccines.
- Benefits
The technology enhances RNA stability and functionality, leading to improved performance of RNA-based applications in various fields.
- Potential Commercial Applications
The technology could be valuable in pharmaceuticals, biotechnology, and research industries for developing novel RNA-based products.
- Possible Prior Art
One potential prior art could be the use of chemical modifications to enhance RNA stability and functionality in various applications.
---
- Unanswered Questions
- How does this technology compare to existing methods for RNA modification and stabilization?
This article does not provide a direct comparison with existing methods or technologies in the field of RNA modification and stabilization.
- What are the potential limitations or challenges associated with the use of acylimidazoles, sulfonyltriazoles, or sulfonylimidazoles for RNA modification?
The article does not discuss any potential limitations or challenges that may arise from using these specific compounds for RNA modification.
Original Abstract Submitted
Compositions and methods are provided for the reversible modification of RNA to enhance RNA in-solution and enzymatic stability by reaction with acylimidazoles, sulfonyltriazoles, or sulfonylimidazoles. 2′-OH acylation protects RNA from hydrolytic and enzymatic degradation. Water-soluble organocatalysts can accelerate the reversal of acylation adducts and functionally restore RNAs, alternatively the acylation is spontaneously reversed in a cellular environment. Chemically tuned 2′-OH acylation can be spontaneously released in cells to restore RNA biological functions including translation. mRNA can be selectively modified at the 2′-OH of poly(A)-tail for enhanced in-cell stability and enhanced total protein output.
